| MNSV p42与寄主因子3-HIBADH互作 |
| |
| 引用本文： | 吴会杰,彭斌,康保珊,古勤生.MNSV p42与寄主因子3-HIBADH互作[J].植物病理学报,2021,51(4):583-591. |
| |
| 作者姓名： | 吴会杰 彭斌 康保珊 古勤生 |
| |
| 作者单位： | 中国农业科学院郑州果树研究所,郑州 450009 |
| |
| 基金项目： | 国家青年科学基金项目(31701941);河南省基础与前沿技术研究项目(152300410231);国家西甜瓜产业技术体系病毒防控岗(CARS-26-13);中央级公益性科研院所基本科研业务费专项(No.1610192020501) |
| |
| 摘 要： | 甜瓜坏死斑点病毒(melon necrotic spot virus,MNSV)是侵染甜瓜的重要病毒之一,为害严重。为了明确MNSV的致病机制,本研究以pGBK-p42为诱饵载体,利用Mating的方法从感染MNSV的甜瓜cDNA文库中筛选出6个与p42互作的寄主因子,进一步利用酵母双杂交系统、双分子荧光互补技术(Bimolecular fluorescence complementation,BiFC)等验证p42与寄主因子3-羟基异丁酸脱氢酶(3-Hydroxyisobutyrate dehydrogenase-like 1, 3-HIBADH)的互作,并明确了p42 蛋白的s-domain是与3-HIBADH互作的关键区域。此外,利用激光共聚焦显微镜观察发现p42 s-domain与3-HIBADH作用改变了3-HIBADH蛋白在烟草表皮的亚细胞定位,进一步证实p42可与3-HIBADH发生相互作用。该结果为阐明甜瓜响应MNSV侵染的互作机制奠定了基础。
|
| 关 键 词： | 甜瓜坏死斑点病毒 外壳蛋白p42 寄主因子 3-羟基异丁酸脱氢酶 互作 |
| 收稿时间： | 2020-06-03 |
Interaction between melon necrotic spot virus p42 and host factor 3-Hydroxyiso-butyrate dehydrogenase-like 1 |
| |
| WU Hui-Jie,PENG Bin,KANG Bao-shan,GU Qin-sheng.Interaction between melon necrotic spot virus p42 and host factor 3-Hydroxyiso-butyrate dehydrogenase-like 1[J].Acta Phytopathologica Sinica,2021,51(4):583-591. |
| |
| Authors: | WU Hui-Jie PENG Bin KANG Bao-shan GU Qin-sheng |
| |
| Institution: | Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China |
| |
| Abstract: | Melon necrotic spot virus(MNSV)is one of the main viruses infecting melon in China.Little is known about the mechanism of interaction between MNSV and the host factors. In this study, we screened the melon host factors interacting with p42 of MNSV and further verified the interaction between them. The bait vector pGBK-p42 was constructed to screen the host factors, the results showed that six host factors could interact with p42 , the interaction between p42 and host factor 3-Hydroxyisobutyrate dehydrogenase- like 1(3-HIBADH) was further confirmed by yeast two-hybrid system. The specific interaction between p42-s domain and 3-HIDBADH was confirmed by yeast two-hybrid system, BiFC and co-localization assays.The results of gene co-localization showed that the location of the 3-HIBADH gene was changed because of the interaction with p42-s domain. A number of small particles were formed in the cytoplasm which further indicated that p42 interacted with 3-HIBADH. The results could promote further understanding the mechanism of interaction between host factors and MNSV. |
| |
| Keywords: | melon necrotic spot virus coat protein p42 host factor 3-Hydroxyisobutyrate dehydrogenase-like l interaction |
|
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
