| 九龙藏黄牛和九龙牦牛β-酪蛋白基因型分析 |
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| 引用本文： | 赵梦波,金素钰,黄林,郑玉才.九龙藏黄牛和九龙牦牛β-酪蛋白基因型分析[J].中国乳业,2021,0(9):120-124. |
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| 作者姓名： | 赵梦波 金素钰 黄林 郑玉才 |
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| 作者单位： | 西南民族大学畜牧兽医学院,四川成都 610041 |
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| 基金项目： | 研究生创新型科研项目（CX2020SP104） |
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| 摘 要： | 为比较九龙藏黄牛和九龙牦牛β-酪蛋白（β-CN）的遗传变异体,试验采用酸性尿素聚丙烯酰胺凝胶电泳分析了九龙藏黄牛（n=42）和九龙牦牛（n=17）β-CN的基因型。结果表明,在九龙藏黄牛、九龙牦牛的β-CN中共检测到4 种等位基因,包括A1、A2、B、C,其中在九龙藏黄牛中有7 种基因型：A1A1、A1A2、BB、A1B、A2B、A1C、BC,优势等位基因为A1（频率0.702 4）,优势基因型为A1A1（频率0.547 6）;在九龙牦牛样本中有2 种基因型：A1A2、A2A2,优势等位基因为A2（频率0.764 7 ）。试验表明,九龙藏黄牛与九龙牦牛β-CN均表现出多态性,但优势等位基因明显不同。
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| 关 键 词： | 藏黄牛 牦牛 β-酪蛋白 酸性尿素电泳 基因型 |
Genotyping on β-caseins of Jiulong Tibetan Yellow Cattle and Jiulong Yak |
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| ZHAO Mengbo,JIN Suyu,HUANG Lin,ZHENG Yucai.Genotyping on β-caseins of Jiulong Tibetan Yellow Cattle and Jiulong Yak[J].China Dairy,2021,0(9):120-124. |
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| Authors: | ZHAO Mengbo JIN Suyu HUANG Lin ZHENG Yucai |
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| Institution: | College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu Sichuan 610041 |
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| Abstract: | The β-casein （β-CN） variants were identified in Jiulong Tibetan Yellow cattle and Jiulong yak by acid urea polyacrylamide gel electrophoresis. The results indicated that four alleles, including A1, A2, B and C, were detected in β-CN of Jiulong Tibetan Yellow cattle and Jiulong yak. There were 7 genotypes in Jiulong Tibetan yellow cattle： A1A1, A1A2, BB, A1B, A2B, A1C and BC, the dominant allele was A1 （the frequency was 0.702 4）, and the dominant genotype was A1A1 （the frequency was 0.547 6）. There were two genotypes in Jiulong yak： A1A2 and A2A2, and the dominant allele was A2 （the frequency was 0.7647）. This study showed that the β-CN polymorphisms were found in both Jiulong Tibetan yellow cattle and Jiulong yak, but the dominant alleles were significantly different. |
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| Keywords: | Tibetan yellow cattle Yak β-casein acid urea electrophoresis genotype |
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