|
|||||
|
|
| 甘肃陇南感病小檗在小麦条锈病发生中起提供(初始)菌源作用的直接证据 | |
| 引用本文： | 刘尧,陈晓云,马雲,孟总彦,王凤林,杨小江,陈雪峰,李小梅,康振生,赵杰.甘肃陇南感病小檗在小麦条锈病发生中起提供(初始)菌源作用的直接证据[J].植物病理学报,2021,51(3):366-380. |
| 作者姓名： | 刘尧 陈晓云 马雲 孟总彦 王凤林 杨小江 陈雪峰 李小梅 康振生 赵杰 |
| 作者单位： | 西北农林科技大学植物保护学院,杨凌 712100; 甘肃省陇南市农业技术推广总站, 武都 746000 |
| 基金项目： | 国家重点研发计划(2018YFD0200500);陇南市科技计划项目(2018-03);陕西省科技计划重点项目(2020JZ-15);中央高校基本科研业务费专项资金资助(2452019046) |
| 摘 要： | 陇南是中国小麦条锈菌易变区、小麦条锈病的常发流行区和防治的关键地区。明确陇南小麦条锈菌转主寄主小檗在小麦条锈病发生中的作用,对阐释该地区小麦条锈菌新小种产生的来源和指导小麦条锈病的综合防控具有重要意义。本研究从陇南春季自然受锈菌侵染的堆花小檗及其邻近的小麦上分离获得小麦条锈菌菌系,19个来自发病小檗的单夏孢子堆菌系在中国小麦条锈菌鉴别寄主上产生5个不同毒力类型VP1~VP5,均为新小种;在近等基因系和载体品种组成的鉴别寄主上产生17个不同毒力类型。29个来自邻近发病小檗的小麦上条锈菌菌系在中国小麦条锈菌鉴别寄主上产生了10个不同的毒力类型VP1~VP10,均为新小种;在近等基因系和载体品种组成的鉴别寄主上产生了24个不同的毒力类型。两个小麦条锈菌群体享有共同的毒力类型VP1~VP5,同时来自发病小檗上菌系的一些毒力类型不同于小麦上的毒力类型。主坐标分析(PCoA)和毒性表型聚类分析表明来自于自然受锈菌侵染小檗及其邻近小麦上的小麦条锈菌存在菌源交流。综合研究表明在自然条件下,陇南小麦条锈菌在野生感病小檗上进行有性生殖是常年发生的,感病小檗在新小种产生和在陇南小麦条锈病的发生中起提供菌源的作用。因此,在陇南小麦条锈病的综合治理策略上加强对小檗的处理,降低新小种产生速率和品种抗性丧失速率,从而延长品种使用年限,同时减少新小种向东部广大麦区传播,保障小麦生产安全。 |
| 关 键 词： | 小麦条锈病 小檗 有性生殖 毒性变异 病害流行 |
| 收稿时间： | 2020-06-19 |
Evidence of roles of susceptible barberry in providing (primary) inocula to trigger stripe rust infection on wheat in Longnan,Gansu |
|
| LIU Yao,CHEN Xiao-yun,MA Yun,MENG Zong-yan,WANG Feng-lin,YANG Xiao-jiang,CHEN Xue-feng,Li Xiao-mei,KANG Zhen-sheng,ZHAO Jie.Evidence of roles of susceptible barberry in providing (primary) inocula to trigger stripe rust infection on wheat in Longnan,Gansu[J].Acta Phytopathologica Sinica,2021,51(3):366-380. | |
| Authors: | LIU Yao CHEN Xiao-yun MA Yun MENG Zong-yan WANG Feng-lin YANG Xiao-jiang CHEN Xue-feng Li Xiao-mei KANG Zhen-sheng ZHAO Jie |
| Institution: | State Key Laboratory of Crop Stress Biology for Arid Area, College of Plant Protection, Northwest A&F University, Yangling 712100, China; Extension Station of Agricultural Technology of Longnan, Wudu 746000, China |
| Abstract: | Longnan, located in southern Gansu, is a virulence variable region (hotspot) for the wheat stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst), a frequent epidemic region for wheat stripe rust, and an important area for management of the disease in China. Determination of the role of barberry (Berberis) as alternate host for Pst in occurrence of wheat stipe rust in Longnan will be beneficial for understanding origin of new races of the pathogen and guiding integrated management of the disease. In the present study, Pst isolates were recovered from naturally rusted barberry (B. aggregata) and wheat plants in field adjacent to the diseased barberry bushes in spring respectively. Nineteen Pst single-uredinium (SU) isolates from the rusted barberry produced 5 virulence patterns (VPs) of VP1-VP5 based on tests on the routine classical differential hosts that are used to differentiating Chinese Pst races, indicating that all of the 19 Pst SU isolates of 5 VPs were new. The 19 Pst SU isolates generated 17 various VPs on a set of combined differentials consisting of near isogenic lines (NILs) and wheat genotypes carrying Yr genes. Twenty nine SU Pst isolates from wheat fields gave rise to 10 VPs of VP1-VP10, identifying that all of them were new races. The 29 SU Pst isolates showed 24 different VPs on the set of the same combined differentials as mentioned the above. Comparisons between the two Pst populations showed that the shared pathotypes VP1-VP5 were shared, and that virulence patterns of some of Pst isolates from the diseased barberry were different from those from wheat fields. Analyses using principal coordinate analysis and clustering of phenotypes based on virulence on the combined differentials indicated the existence of gene flow between both populations from the naturally rusted barberry and wheat fields close to the diseased barberry bushes. Altogether, the results indicated that under natural conditions occurrence of Pst sexual reproduction on wild susceptible Berberis species in Longnan is routine, and that susceptible Berberis species play in a role in providing aeciospores-derived from barberry plants as inocula to cause stripe rust infection on wheat in this area. Therefore, treatment of barberry bushes should be considered in integrated management of wheat stripe rust in Longnan. This strategy will be apt to reduce rates of generating new races of Pst and breakdown of the resistance of wheat cultivars against stripe rust, extending utilization of stripe rust-resistant wheat cultivars and decreasing spread of new races to wheat production areas in eastern China for ensuring safe production of wheat. |
| Keywords: | wheat stripe rust Berberis sexual reproduction virulence variation disease epidemic |
| 点击此处可从《植物病理学报》浏览原始摘要信息 | |
| 点击此处可从《植物病理学报》下载免费的PDF全文 | |