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| 浙江甘薯蔓枯病病原菌鉴定 | |
| 引用本文： | 谢昀烨,王会福,应俊杰,方丽,王连平,王汉荣.浙江甘薯蔓枯病病原菌鉴定[J].植物病理学报,2021,51(3):441-445. |
| 作者姓名： | 谢昀烨 王会福 应俊杰 方丽 王连平 王汉荣 |
| 作者单位： | 浙江省农业科学院植物保护与微生物研究所,杭州 310021; 台州市农科院,临海 317000; 仙居县植物保护检疫站,仙居 317300 |
| 基金项目： | 浙江省农业科学院地方科技合作项目(TZ2018009) |
| 摘 要： | |
| 收稿时间： | 2020-05-26 |
Identification of pathogen Diaporthe spp. associated with gummy stem blight of Ipomoea batatas in Zhejiang |
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| XIE Yun-ye,WANG Hui-fu,YING Jun-jie,FANG Li,WANG Lian-ping,WANG Han-rong.Identification of pathogen Diaporthe spp. associated with gummy stem blight of Ipomoea batatas in Zhejiang[J].Acta Phytopathologica Sinica,2021,51(3):441-445. | |
| Authors: | XIE Yun-ye WANG Hui-fu YING Jun-jie FANG Li WANG Lian-ping WANG Han-rong |
| Institution: | Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China; Taizhou Academy of Agricultural Sciences,Linhai 317000,China; Xianju County Plant Protection and Quarantine Station,Xianju 317300,China |
| Abstract: | Recent years, gummy stem blight caused by Diaporthe spp. is an important disease on Ipomoea batatas. In this study, infected plant tissues, collected from Ningxi, Taizhou, were used for isolating pathogens from the gummy stem. Three pure strains with similar cultural characteristics were isolated, tested for their pathogenicity against stems of I. batatas, and re-isolated from the tissue inoculated, and Koch's postulate was fulfilled. The rRNA intergenic spacer (ITS) region of these isolates was amplified and the sequences were used for the phylogenetic analysis. The aliment result showed that the sequences (MT107556, MT107557 and MT107558) of three strains were 100% similarity with that of D. batatas CBS 122.21. These isolates were identified as D. batatas based on the morphological and molecular characteristics. Therefore, gummy stem blight on Ipomoea batatas was caused by D. batatas in Zhejiang province. Meanwhile, this study firstly showed that the stroma of D. batatas was coralloid. |
| Keywords: | Diaporthe sweet potato stroma morphological characteristics phylogenetic tree |
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