| 手性除草剂2甲4氯丙酸对映体在拟南芥中吸收转运及亚细胞分布的差异研究 |
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| 引用本文： | 常江海,郭维,郑姚颖,张素芬,叶庆富.手性除草剂2甲4氯丙酸对映体在拟南芥中吸收转运及亚细胞分布的差异研究[J].核农学报,2021,35(3):681-687. |
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| 作者姓名： | 常江海 郭维 郑姚颖 张素芬 叶庆富 |
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| 作者单位： | 浙江大学原子核农业科学研究所/农业部核农学重点实验室,浙江杭州310029;浙江大学原子核农业科学研究所/农业部核农学重点实验室,浙江杭州310029;浙江大学原子核农业科学研究所/农业部核农学重点实验室,浙江杭州310029;浙江大学原子核农业科学研究所/农业部核农学重点实验室,浙江杭州310029;浙江大学原子核农业科学研究所/农业部核农学重点实验室,浙江杭州310029 |
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| 基金项目： | 国家基金面上项目(11675142),国家重点研发计划子课题(2016YFD0200201) |
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| 摘 要： | 为探明不同手性构型的2甲4氯丙酸(mecoprop,MCPP)在双子叶植物中的吸收、代谢等差异,以手性14C-MCPP对映体为示踪剂,野生哥伦比亚型拟南芥为受试植物,研究实验室条件下不同手性构型的MCPP在拟南芥中的吸收转运与亚细胞分布特征。结果表明,拟南芥对14C-MCPP 2种对映体的吸收效率分别为90.45%(14C-R-MCPP)和85.75%(14C-S-MCPP),二者差异显著。施药后,2种构型的14C-MCPP均易向下运转,其中14C-R-MCPP在拟南芥植株中的转运过程更持久,转运到植株其他部位的比率相对更高。2种构型14C-MCPP在标记下位达到平衡时,14C-R-MCPP的分布比率为14C-S-MCPP的2.5倍。亚细胞分布方面,在拟南芥标记叶位细胞中各组分的分布比率均为胞浆>细胞壁>细胞器,在施药后144 h,14C-S-MCPP在细胞壁的分布比率较14C-R-MCPP高,分别为39.60%和28.74%,表明拟南芥叶组织细胞壁对14C-S-MCPP具有更强的固定能力;而在细胞器中,14C-R-MCPP的分布比率(10.55%)较14C-S-MCPP(4.11%)高,表明14C-R-MCPP相较于14C-S-MCPP更易富集于叶组织细胞的细胞器中,在同施药浓度下与靶标蛋白的接触频率更高。综上可知,手性激素型除草剂MCPP在双子叶植物模式植物拟南芥中的运转和分布存在显著的对映体选择性差异,映证了R-MCPP从吸收、转运、亚细胞分布的层面而言相较于其对映体S-MCPP具有更强的除草活性。
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| 关 键 词： | 2甲4氯丙酸 拟南芥 吸收 转运 亚细胞分布 |
| 收稿时间： | 2019-01-24 |
Uptake,Translocation and Subcellular Distribution of Mecoprop Enantiomers in Arabidopsis thaliana |
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| CHANG Jianghai,GUO Wei,ZHENG Yaoying,ZHANG Sufen,YE Qingfu.Uptake,Translocation and Subcellular Distribution of Mecoprop Enantiomers in Arabidopsis thaliana[J].Acta Agriculturae Nucleatae Sinica,2021,35(3):681-687. |
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| Authors: | CHANG Jianghai GUO Wei ZHENG Yaoying ZHANG Sufen YE Qingfu |
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| Institution: | Institute of Nuclear-Agricultural Science, Zhejiang University/Key Laboratory of Ministry of Agriculture, Nuclear Agricultural Science, Hangzhou, Zhejiang 310029 |
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| Abstract: | Mecoprop is a chiral auxin herbicide possessing one chiral center and two enantiomers and R-MCPP exhibits more significant herbicidal activity than S-MCPP. In order to Fig out the differences of absorption and metabolism of MCPP enantiomers in dicotyledons, the experiment an absorption, translocation and subcellular distribution of 14C-MCPP enantiomers in Arabidopsis thaliana had been carried out under laboratory conditions with 14C-MCPP enantiomers as tracer agent and Arabidopsis thaliana as plant material. The result showed that the plant took up the two enantiomers differently, with the absorption ratio of 85.75%(S-MCPP) and 90.45%(R-MCPP). As for translocation, R-MCPP had an increased translocation capacity with longer translocation time before equilibrium state and higher translocation ratio. Once reaching the balance, the translocation ratio of R-MCPP was 1.5 times greater than that of S-MCPP. Subcellular distribution test indicated that more of two MCPP enantiomers existed in the cytoplasm, followed by in cytoderm and organelle. The distribution ratio of S-MCPP (39.60%) in cytoderm was higher than that of R-MCPP (28.74%), indicating higher fixation of S-MCPP in cytoderm. Whereas the R-MCPP in organelle was 10.55% higher than 4.11% for S-MCPP, indicating that some herbicidal receptors were easier to be exposed to R-MCPP. According to the above results, the research, to a certain extent, elucidated the significant enantioselectivity of chiral MCPP on herbicidal activity in model dicotyledon Arabidopsis thaliana. |
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| Keywords: | MCPP enantiomers Arabidopsis thaliana absorption translocation subcellular distribution |
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