| 香蕉秸秆不同还田模式对土壤微生物群落的影响 |
| |
| 引用本文： | 孙鹏,刘满意,王蓓蓓.香蕉秸秆不同还田模式对土壤微生物群落的影响[J].热带生物学报,2021,12(1):57-62. |
| |
| 作者姓名： | 孙鹏 刘满意 王蓓蓓 |
| |
| 作者单位： | 海南大学热带作物学院,海口570228 |
| |
| 基金项目： | 国家重点研发计划（2017YFD0202101）；国家自然科学基金项目（41867006） |
| |
| 摘 要： | 采用土壤室内培养的方法，以香蕉秸秆不同还田模式为试验唯一变量因素，设置3个处理（无秸秆还田CK；将香蕉秸秆覆盖到土壤表层，作为秸秆自然还田处理F；将土壤与香蕉秸秆彻底混合，作为秸秆掩埋还田处理T），分析香蕉秸秆不同还田模式下的微生物群落差异，以期揭示香蕉秸秆的不同还田模式对土壤微生物的影响。结果表明:T、F处理与CK处理相比，真菌门中Basidiomycota相对丰度分别增加1.3%和2.8%；细菌门中Acidobacteria相对丰度分别增加5.1%和1.1%，Actinobacteria的相对丰度分别增加1.9%和3.2%。处理间Fusarium相对丰度大小顺序为CK>F>T。处理间细菌群落的丰富度（SChao1）和多样性（HShannon）大小顺序均为T>F>CK，真菌群落并无明显差异。聚类分析表明，T处理土壤微生物群落结构与CK处理明显不同。主坐标分析和OTU数量分布结果表明，T处理土壤微生物群落结构与F处理相近。本研究结果显示，T处理增加了土壤细菌群落的丰富性与多样性，改变了土壤微生物的群落结构，同时提高了具有分解功能的微生物分类属的相对丰度，并显著降低了病原菌的相对丰度。
|
| 关 键 词： | 香蕉秸秆 微生物群落结构 微生物群落多样性 微生物群落丰富度 MiSeq sequencing 秸秆还田模式 |
| 收稿时间： | 2020-11-17 |
Effects of Fresh Harvested Banana Foliage on the Soil Microbial Community Structures |
| |
| Institution: | College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China |
| |
| Abstract: | Soil samples collected from a banana plantation in Hongxing Farm, Lingao, Hainan, were potted, and fresh harvested foliage (leaves and pseudostems) collected from the banana plantation were grounded and returned to the potted soil for indoor culture to analyze the difference of soil microbial communities in the potted soil for revelation of the effects of soil returning of the fresh harvested foliage on soil microorganism. Three treatments were arranged: soil without banana foliage (control, CK); soil mulched with banana foliage (F); soil mixed with banana foliage (T). The results showed that the relative abundance of fungal phylum Basidiomcota in the T and F treatments increased by 1.3% and 2.8%, respectively, as compared with the CK, while the relative abundance of bacterial phylum Acidobacteria increased by 5.1% and 1.1%, respectively, and that of Actinobacteria increased by 1.9%, 3.2%, respectively. The relative abundance of Fusarium between treatments was in the order of CK>F>T. The bacterial community richness (SChao1) and diversity (HShannon) between the treatments were in the order of T>F>CK, and no difference was observed in fungal communities between the treatments. Cluster analysis showed that the structure of soil microbial community in the treatment T was significantly different from that of CK. Principal coordinate analysis and OTU distribution showed that the structure of soil microbial community in the treatment T was similar to that in the treatment F. It is concluded that the treatment T increased the richness and diversity of soil bacterial community, changed the structure of soil microbial community, improved the relative abundance of microorganisms that have a decomposition function, and significantly reduced the relative abundance of pathogen Fusarium. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《热带生物学报》浏览原始摘要信息 |
| 点击此处可从《热带生物学报》下载免费的PDF全文 |
|
