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| 虎耳草黑斑病的病原分离鉴定及生物学特性研究 | |
| 引用本文： | 张国俊,梁霜,丁海霞,赵致,李忠.虎耳草黑斑病的病原分离鉴定及生物学特性研究[J].植物病理学报,2021,51(3):451-455. |
| 作者姓名： | 张国俊 梁霜 丁海霞 赵致 李忠 |
| 作者单位： | 贵州大学农学院,贵阳 550025; 贵州省药用植物繁育与种植重点实验室,贵阳 550025 |
| 基金项目： | 贵州中药材创新成果应用,中央引导地方科技发展专项资金项目(黔科中引地[2018]4018);科技部科技惠民计划项目(2013GS520101-08) |
| 摘 要： | |
| 收稿时间： | 2020-07-16 |
Identification and biological characteristics of pathogen causing black spot disease of Saxifraga stolonifera (L.)Meerb. |
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| ZHANG Guo-jun,LIANG Shuang,DING Hai-xia,ZHAO Zhi,LI Zhong.Identification and biological characteristics of pathogen causing black spot disease of Saxifraga stolonifera (L.)Meerb.[J].Acta Phytopathologica Sinica,2021,51(3):451-455. | |
| Authors: | ZHANG Guo-jun LIANG Shuang DING Hai-xia ZHAO Zhi LI Zhong |
| Institution: | Agriculture College of Guizhou University, Guiyang 550025, China; Guizhou Key Laboratory of Propagation and Cultivation on Medicinal Plants, Guizhou University, Guiyang 550025, China |
| Abstract: | To identify the pathogen causing black spot disease of Saxifraga stolonifera (L.) Meerb. in the base of Chinese medicinal material (Niudachang Town, Shibing County, Guizhou), we took tissue separation method and tested its pathogenicity according to the Koch's rule. Combined with morphology and multi-gene (ITS, LSU, RPB2) phylogenetic analysis, we identified Hu42 colony as the pathogen causing the disease. Hu42 colony exhibited highly similar as Leptosphaerulina fungus with an appearance of nearly round, gray-black on the front, light brown on the edge, black on the back, its mycelium was light brown with septal branches and did not produce ascus or ascospores. Based on the phylogenetic tree constructed by multiple genes, the strain was clustered together with Leptosphaerulina americana strain at a support rate of 88%. It was identified as L. americana based on the morphological characteristics and the molecular biology. Moreover, we found that the suitable cultivation conditions of Hu42 are 25℃-28℃, 12 h alternating light and dark, the pH at 6-7, soluble starch and yeast extract powder as the carbon and nitrogen sources, and PDA, PSA or beef extract peptone as medium. This study is the first report on black spot disease caused by L. americana on S. stolonifera (L.) Meerb. |
| Keywords: | Saxifraga stolonifera(L )Meerb black spot disease identification Leptosphaerulina americana biological characteristics |
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