| 小鹅瘟地方株的分离鉴定及致病力试验 |
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| 引用本文： | 陈晓月,赵承辉,刘爽.小鹅瘟地方株的分离鉴定及致病力试验[J].沈阳农业大学学报,2007,38(3):410-412. |
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| 作者姓名： | 陈晓月 赵承辉 刘爽 |
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| 作者单位： | 1. 沈阳农业大学,畜牧兽医学院,沈阳,110161
2. 辽宁省农业技术学校,沈阳,110161 |
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| 摘 要： | 通过对当地鹅场发生的小鹅瘟疑似病例的病变肝脏进行病毒分离,将具有病变的肝脏加hanks液进行研磨后,低温反复冻融3次,除菌过滤后接种12日龄的鹅胚。收集死亡鹅胚的尿囊液,初步分离出鹅细小病毒,经电镜观察、琼脂扩散试验、血清保护试验鉴定为鹅细小病毒。动物回归试验表明,该病毒的潜伏期为5d,病程为1～3d,病死率达100%,对雏鹅半数致死量(LD50)为0.1mL 10-4.5稀释的病毒液。
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| 关 键 词： | 鹅 细小病毒 分离鉴定 |
| 文章编号： | 1000-1700（2007）03-0410-03 |
| 修稿时间： | 2006-04-04 |
Identification and Pathogenesis of GPV Wild Strain Isolated |
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| CHEN Xiao-yue,ZHAO Cheng-hui,LIU Shuang.Identification and Pathogenesis of GPV Wild Strain Isolated[J].Journal of Shenyang Agricultural University,2007,38(3):410-412. |
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| Authors: | CHEN Xiao-yue ZHAO Cheng-hui LIU Shuang |
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| Institution: | 1. College of Animal science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China; 2. Liaoning Agricultural Science and Technology School, Shenyang 110161, China |
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| Abstract: | A strain GPV was isolated successfully in the case of GPD. It was determined to be a goose parvovirus by VN, AGP tests. The results of regressive tests of GPV isolated strain showed that latent period was 5 days, lasting 1-3 days after infected, and lethal rate was 100%. LDso of the GPV was 0.1mL 10^-45 diluted virus liqued. |
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| Keywords: | goose parvovirus identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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