Estimating the fatty acid composition of the oil in intact-seed rapeseed (Brassica napus L.) by near-infrared reflectance spectroscopy |
| |
| Authors: | Leonardo Velasco Heiko C Becker |
| |
| Institution: | 1. Institut für Pflanzenbau und Pflanzenzüchtung, Georg-August-Universit?t, Von-Siebold-Str. 8, D-37075, G?ttingen, Germany
|
| |
| Abstract: | The objective of this work was to evaluate the potential of near-infrared reflectance spectroscopy (NIRS) as a rapid method
to estimate the fatty acid composition of the oil in intact-seed samples of rapeseed. A total of 549 samples (3 g intact seed)
from selected mutant and breeding lines were scanned by NIRS, and 220 of them were selected and scanned again by using two
different adapters, which reduced the sample size to 300 and 60 mg, respectively. Selected samples were analysed by gas liquid
chromatography and calibration equations for individual fatty acids were developed. Calibrations for oleic, linoleic, linolenic,
and erucic acid were highly accurate, with values of r2 in cross validation from 0.95 to 0.98 (samples of 3 g), from 0.93 to 0.97 (300 mg), and from 0.84 to 0.96 (60 mg). Calibrations
for palmitic and stearic acid were less accurate, with values of r2 in cross validation always lower than 0.8, probably because of the narrow range available for these fatty acids. The accuracy
of the calibration equations for eicosenoic acid was very low (r2 = 0.69 in 3 g samples), although improved equations were developed (r2 from 0.78 to 0.91) when the relationship between erucic and eicosenoic acid was taken into account. We conclude that NIRS
is a powerful technique to estimate the fatty acid composition of the oil in rapeseed, provided that samples covering a wide
range of fatty acid levels are available, with the advantage that such estimation is possible with few additional costs when
NIRS is used for the determination of other seed quality traits.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
| Keywords: | Brassica napus fatty acid composition NIRS rapeseed reflectance spectroscopy |
| 本文献已被 SpringerLink 等数据库收录！ |
|
