| 卷荚相思组培快繁技术研究 |
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| 引用本文： | 汪长水.卷荚相思组培快繁技术研究[J].福建林业科技,2009,36(3). |
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| 作者姓名： | 汪长水 |
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| 作者单位： | 福建省林业科技试验中心,福建南靖,363600 |
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| 基金项目： | 福建省林业厅科研项目"卷荚相思和厚荚相思快繁技术及推广应用研究" |
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| 摘 要： | 以卷荚相思优树的带芽茎为外植体,进行初代培养、继代苗增殖培养、生根培养和组培苗移栽等组培快繁技术研究,结果表明,外植体初代培养最合适流程为:树冠中上部穗条经70%酒精消毒处理40 s后,再用0.1%HgCl2溶液消毒处理8min,萌动率高达35.7%;继代培养的最佳培养基为:改良MS+6-BA 0.8 mg.L-1+IAA 0.5 mg.L-1+蔗糖30 g.L-1,增殖倍数最高达5.2倍;生根培养以继代芽苗长1.1~1.4 cm,1/2MS+IBA 1.5 mg.L-1+ABT1#0.2 mg.L-1+NAA0.2 mg.L-1+蔗糖30 g.L-1培养基最佳,生根率最高达97.8%;组培苗移栽的最佳基质为红心土+泥炭土(3∶1),成活率达90%。
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| 关 键 词： | 卷荚相思 初代培养 继代培养 生根培养 移栽 |
Study on Technology of Tissue Culture and Rapid Propagation of Acacia cincinnata |
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| WANG Chang-shui.Study on Technology of Tissue Culture and Rapid Propagation of Acacia cincinnata[J].Journal of Fujian Forestry Science and Technology,2009,36(3). |
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| Authors: | WANG Chang-shui |
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| Institution: | WANG Chang-shui(Fujian Provincial Forestry Technological Test Center,Nanjing 363600,Fujian,China) |
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| Abstract: | The tissue culture and rapid propagation technology researches of generation-beginning culture,the generation-continuing seedling multiplication culture,rooting culture and culture seedling transplanting were conducted with Acacia cincinnata plus tree stem with buds as explant.The results revealed: the suitable flow of the explant generation-beginning culture was following: the spikes on the mid and upper locations of crown were treated by 70% of alcohol for 40s,then were sterilized with 0.1% of HgCl2 for 8... |
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| Keywords: | Acacia cincinnata generation-beginning culture rooting culture transplant |
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