| 黑粒小麦的选育及其抗氧化特性 |
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| 引用本文： | 张东阳,李集临,张杰.黑粒小麦的选育及其抗氧化特性[J].中国农学通报,2010,26(5):93-96. |
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| 作者姓名： | 张东阳 李集临 张杰 |
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| 作者单位： | 哈尔滨师范大学,哈尔滨,150025 |
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| 基金项目： | 哈师大博士科研启动基金项目"黑粒小麦色素的分子遗传研究" |
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| 摘 要： | 摘要：本文选育的黑粒小麦为黑皮黑胚乳，含有丰富的水溶性黑色素的小麦，国内外对黑色素作了一定的开发研究，但黒粒小麦清除二苯基-2-苦肼基自由基的研究还很少。本研究以普通小麦和抗坏血酸为对照，通过DPPH 法测定黑粒小麦色素粗提物体外抗氧化、清除自由基能力。研究结果表明:黑粒小麦黑色素粗提物具有呈量效关系的抗氧化活性，其清除自由基能力远大于普通小麦，但小于抗坏血酸。普通小麦、黑粒小麦3-2、3-3、3-4、3-11、3-13、3-33、3-36、3-37、3-39、10-38和Vc清除50% DPPH 自由基的有效浓度（EC50）分别为689.05、39.89、71.51、58.57、48.13、33.99、105.27、62.15、30.97、35.15、35.09、0.196 mg/ml,其自由基清除能力AE 分别为0.15×10-2、2.51×10-2、1.4×10-2、1.7×10-2、2.08×10-2、2.94×10-2、0.95×10-2、1.61×10-2、3.23×10-2、2.84×10-2、2.85×10-2、510×10-2。这些为进一步研究、开发和利用黒粒小麦黑色素奠定理论基础。
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| 关 键 词： | 烤烟 烤烟 烘烤 密集烤房 |
| 收稿时间： | 2009/10/14 0:00:00 |
| 修稿时间： | 2009/11/23 0:00:00 |
Study on the Breeding and Anti-aging Effect of Black-grained Wheat |
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| Zhang Dongyang,Li Jilin,Zhang Jie.Study on the Breeding and Anti-aging Effect of Black-grained Wheat[J].Chinese Agricultural Science Bulletin,2010,26(5):93-96. |
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| Authors: | Zhang Dongyang Li Jilin Zhang Jie |
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| Institution: | (Life College, Harbin Normal University, Harbin 150025) |
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| Abstract: | Our Black-grained Wheat contains black endosperm and rich water-soluble pigment, which had been studied both at home and abroad. There was almost no study on free radical scavenging effect of these pigments in Black-grained Wheat by DPPH.＇ Using Chinese Spring and Vc as the control, We evaluated free radical scavenging and anti-aging effect of pigment crude extract in black-grained wheat by DPPH. The results showed that pigment in Black-grained Wheat had close-dependent effect on free radical scavenging. The ability of free radical scavenging and anti-aging of the pigment crude extracted from Black-grained Wheat was greater than those of Chinese Spring, but lower than those of Vc. ECs0 （50% the initial DPPH concentration） of Chinese Spring, Black-grained Wheat 3-2, 3-3, 3-4, 3-11, 3-13, 3-33, 3-36, 3-37, 3-39, 10-38 and Vc was 689.05, 39.89, 71.51, 58.57, 48.13, 33.99, 105.27, 62.15, 30.97, 35.15, 35.09, 0.196 mg/mL DPPH respectively, and the antiradical efficiency （AE） was 0.15×10-2、2.51×10-2、1.4×10-2、1.7×10-2、2.08×10-2、2.94×10-2、0.95×10-2、1.61×10-2、3.23×10-2、2.84×10-2、2.85×10-2、510×10-2. All of this lay a foundation for further study, exploit and utilize pigment in Black-grained Wheat. |
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| Keywords: | DPPH |
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