| 相同耐药谱的11株大肠杆菌的脉冲场电泳分型研究 |
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| 引用本文： | 杨玲丽,陈杖榴.相同耐药谱的11株大肠杆菌的脉冲场电泳分型研究[J].中兽医医药杂志,2004,23(4):19-20. |
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| 作者姓名： | 杨玲丽 陈杖榴 |
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| 作者单位： | 华南农业大学兽医学院,广东省兽药研制与安全评价重点实验室,广东,广州,510642 |
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| 摘 要： | 为了了解同一猪场不同来源具相同耐药谱的11株大肠杆菌之间的同源性,以探讨其耐药流行机制,用脉冲场凝胶电泳(PFGE)对同一猪场不同来源具相同耐药谱的大肠杆菌做染色体DNA分型.结果发现分别来自小猪和环境的2株大肠杆菌通过PFGE分型,属于同一个亚型.初步认为不同来源的大肠杆菌来自不同克隆系,同时在动物环境之间可能存在着耐药性的克隆传播.结果表明,PFGE可作为耐药性流行病学调查的一种研究方法.
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| 关 键 词： | 大肠杆茵 脉冲场凝胶电泳 相同耐药谱 |
| 文章编号： | 1000-6354(2004)04-0019-02 |
| 修稿时间： | 2004年2月26日 |
Study on pulsed field gel electrophoresis types of Escherichia coli with the same resistant spectrums |
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| YANG Ling-li,CHEN Zhang-liu.Study on pulsed field gel electrophoresis types of Escherichia coli with the same resistant spectrums[J].Journal of Traditional Chinese Veterinary Medicine,2004,23(4):19-20. |
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| Authors: | YANG Ling-li CHEN Zhang-liu |
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| Abstract: | To study the correlation of strains of Escherichia coli with the same resistant spectrums isolated from different places of one pig industry, and to obtain the knowledge about the molecular epidemiology of resistance. The pulsed field gel electrophoresis (PFGE) was applied to chrosome DNA types of the E.coli with the same resistant spectrums isolated from different places of one pig industry. 2 strains of 6 multi-resistant E.coli were classified into the same subtype, which were from pig and environment respectively. There exists different clone of E.coli in different places, which also empresses PFGE can be used as a method to study the molecular epidemiology of resistance. |
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| Keywords: | Escherichia coli PFGE the same resistant spectrums |
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