First report of Enterocytozoon bieneusi from dairy cattle in Argentina |
| |
| Authors: | Valeria F Del Coco María Alejandra Córdoba Gladys Bilbao Pinto de Almeida Castro Juan Angel Basualdo Mónica Santín |
| |
| Institution: | 1. Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 60 y 120, La Plata 1900, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina;3. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Argentina;4. Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Argentina;5. Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, MD 20705, USA |
| |
| Abstract: | Fecal specimens were obtained from a total of 70 dairy calves less than two months old on 11 municipalities in Buenos Aires, Argentina. After removal of fecal debris by sieving and sucrose flotation, specimens were subjected to PCR to detect the presence of Enterocytozoon bieneusi. PCR revealed a 14.3% of prevalence for E. bieneusi with 10 positive calves from 7 municipalities. Gene sequence analysis conducted in all samples positives by PCR revealed the presence of six genotypes; four previously reported in cattle as well as humans (D, I, J, and BEB4), one never reported in cattle before but previously reported in humans (EbpC), and one novel genotype (BEB10). These results constitute the first molecular characterization of E. bieneusi in Argentina, and suggest a potential risk of zoonotic transmission in this area. |
| |
| Keywords: | Argentina Cattle Genotype Microsporidia PCR |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
