Isolation and identification of select oligosaccharides from commercially produced total invert sugar with a proposed mechanism for their formation |
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| Authors: | Thavarajah Pushparajah Low Nicholas H |
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| Institution: | Department of Applied Microbiology and Food Science, College of Agriculture, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 5A8. |
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| Abstract: | Three disaccharides were isolated and purified from a commercial total invert sugar (TIS). The structures of these compounds were determined by a combination of acid and enzymatic hydrolysis studies, chromatographic comparison to standards, and nuclear magnetic resonance spectroscopy. These carbohydrates were identified as O-alpha-D-glucopyranosyl-(1-->3)-D-fructose (IS1), O-beta-D-fructofuranosyl-(2-->6)-D-glucose (IS2), and O-alpha-D-glucopyranosyl-beta-D-glucopyranoside (IS3). On the basis of these structures a mechanism for the hydrochloric acid catalyzed hydrolysis of sucrose is proposed: protonation of the glycosidic oxygen of sucrose leading to the formation of glucopyranosyl and fructofuranosyl oxonium ions of D-glucose or D-fructose, respectively, followed by nucleophilic attack of these ions by D-glucose or D-fructose at either the alpha- or beta-face. |
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