|
|||||
|
|
| 利用线粒体Cyt b为遗传标记分析重庆地区东方蜜蜂的遗传多样性 | |
| 引用本文： | 于增源,刘意秋,周丹银,赵文正,戴荣国,和绍禹,苗永旺.利用线粒体Cyt b为遗传标记分析重庆地区东方蜜蜂的遗传多样性[J].蜜蜂杂志,2012,32(11):1-3. |
| 作者姓名： | 于增源 刘意秋 周丹银 赵文正 戴荣国 和绍禹 苗永旺 |
| 作者单位： | 1. 云南农业大学东方蜜蜂研究所,云南昆明,650201 2. 重庆市畜牧科学研究院蜜蜂研究所,重庆荣昌,402460 3. 云南农业大学动物科学技术学院,云南昆明,650201 |
| 基金项目： | 国家农业产业技术体系(蜜蜂)CARS-45-kxjl4 |
| 摘 要： | 采用PCR产物直接测序技术对重庆地区117群东方蜜蜂样品的线粒体DNA细胞色素b基因(Cyt b)420 bp长度序列进行了分析。结果显示:在所得到的序列中共检测到11个核苷酸多态位点,其中单一变异位点2个,简约信息位点9个,序列突变率为2.62%,序列T,C,A,G平均含量分别为44%,14.2%,32.9%,8.9%,共定义8种单倍型,单倍型多样度为0.773±0.021,核苷酸多样度为0.003 82±0.000 32,单倍型网络关系图揭示重庆地区东方蜜蜂群体没有明显的遗传分化。 |
| 关 键 词： | 重庆市 东方蜜蜂 遗传多样性 细胞色素b |
Genetic diversity of Apis cerana in Chongqing Using mtDNA Cytochrome b gene Sequences |
|
| Abstract: | The sequences of mtDNA Cytochrome b gene of 120 colonies of Apis cerana collected from Chongqing area were analyzed by the method of PCR with direct se- quencing. The results showed： A total of 8 mitochondrial haplotypes with 1 1 polymorphic sites included 2 singleton polymorphic sites and 9 parsimony informative sites were detected in the samples. The average frequencies of T, C, A and G for the sequences of Apis cerana were 44%, 14.2%, 32.9% and 8.9%, respectively. And haplotype diversity, nu- cleotide diversity, between two different regions were 0.773 ±0.021 and 0.003 82±0.000 32. There was no obvi- ously genetic differentiation in Chongqing indicated by the Network relation. |
| Keywords: | Chongqing Apis cerana genetic diversity cytochome b |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |