Mass propagation of Rauwolfia serpentina L. Benth |
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| Authors: | Salma U Rahman M S M Islam S Haque N Khatun M Jubair T A Paul B C |
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| Institution: | Department of RDDR, Modem Herbal Group, Dhaka 1217, Bangladesh. |
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| Abstract: | A protocol for mass propagation through axillary bud proliferation was established for Rauwolfia serpentina L. Benth. (Apocynaceae). MS medium supplemented with 1.5 mg L(-1) BA and 0.2 mg L(-1) NAA elicited the maximum number of shoots (4 multiple shoots) from nodal explants. These adventitious shoots were best rooted on half strength MS medium supplemented with 1.0 mg L(-1) each of IBA and IAA. The in vitro raised plants were acclimatized in glass house and successfully transplanted to field condition with almost 95% survival. |
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