Method and quantity of liquid formulation of phosphobacteria required for seed inoculation |
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Authors: | Gomathy M Thangaraju M Gunasekaran S Gopal N O Gopal H |
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Institution: | Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Tamil Nadu, Coimbatore-641003, India. |
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Abstract: | The survival of vegetative and sporulated cells of the Bacillus cultures on the seeds of the crop plants was tried in different combinations. One milliliter inoculum with 1 mL adhesive combination or sterile water showed better results followed by 1.5 mL inoculum with 0.5 mL adhesive or sterile water. The population of 5.5x10(5) cfu seed(-1) on black gram, 10.5x10(5) cfu seed(-1) on soybean and 6.5x10(5) cfu seed(-1) on maize were observed after 12 h of incubation in 1 mL sporulated inoculum mixed with 1 mL of rice gruel. The sporulated inoculum along with rice gruel favoured the adherence of the regenerated cells as rice gruel is rich in nutrient content. |
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