| 东北地区部分犊牛感染贾第虫的基因型及基因亚型分析 |
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| 引用本文： | 张静,苏艳,白光彦,王春仁,高依然,韩福松,姜宁,陈启军,尹继刚.东北地区部分犊牛感染贾第虫的基因型及基因亚型分析[J].兽医大学学报,2012(11):1679-1682,1707. |
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| 作者姓名： | 张静 苏艳 白光彦 王春仁 高依然 韩福松 姜宁 陈启军 尹继刚 |
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| 作者单位： | [1]吉林大学人兽共患病研究所,人兽共患病研究教育部重点实验室,吉林长春130062 [2]吉林市丰满区小白山乡兽医站,吉林吉林市132108 [3]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 |
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| 基金项目： | 国家“十一·五”传染病重大专项资助项目（ZX10004011） |
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| 摘 要： | 采用巢式PCR方法鉴定东北部分地区牛源贾第虫的基因型。提取DNA后经巢式PCR扩增TPI基因，扩增产物测序后用BLAST和MEGA4．0软件进行同源性和系统发育分析。结果表明，吉林市和长春分离株属于蓝氏贾第虫的AssemblageE型；大庆分离株中蓝氏贾第虫的AssemblageE和AssemblageA型均存在。
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| 关 键 词： | 贾第虫 基因型 基因亚型 |
Genotype in dentification of Giardia from cattle in part areas of northeast China |
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| Authors: | ZHANG Jing SU Yan BAI Guang-yan WANG Chun-ren GAO Yi-ran HAN Fu-song JIANG Ning CHEN Qi-j un YIN Ji-gang |
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| Institution: | 1. Institute of Zoonosis, J ilin University, Key Laboratory of Zoonosis ,Ministry of Education, Changchun 130062, China ; 2. Xiaobaishan Township Diseases Control Center for Livestock, Jilin City, Jilin 132108, China ; 3. College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang 163319 ,China) |
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| Abstract: | Giardia isolated from feces of naturally infected calves in part areas of northeast China were genotyped by nested PCR. After the genornic DNA of Giardia was extracted, the TPI gene was amplified by nested PCR. The BLAST and MEGA4. 0 were used to analyze their homology and phylogeny. As demonstrated by TPI gene analysis,the Jilin and Changchun isolates belong to Assemblage E of G. larnblia; Assemblage E and A of G. larnblia all existed in Daqing isolates. |
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| Keywords: | Giardia genotype subgenotype |
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