| 云芝液体培养过程中胞外酶与胞外多糖的变化 |
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| 引用本文： | 王俊明,周自云,胡景江,曹支敏.云芝液体培养过程中胞外酶与胞外多糖的变化[J].西北林学院学报,2004,19(2):22-24. |
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| 作者姓名： | 王俊明 周自云 胡景江 曹支敏 |
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| 作者单位： | 西北农林科技大学,生命科学学院,陕西,杨陵,712100; 西北农林科技大学,林学院,陕西,杨陵,712100 |
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| 基金项目： | 高等学校全国优秀博士学位论文作者专项基金项目"秦岭大型真菌物种多样性及其生态功能研究"(200057) |
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| 摘 要： | 研究了云芝在液体培养过程中胞外酶与胞外多糖等相关指标的动态变化.结果表明,在28℃,150 r/min恒温振荡培养条件下,云芝茵丝体生物量在第12 d达到最大值(6.436 g/L);发酵液的pH值在整个液体培养过程中变化不大;胞外蛋白质浓度于第11 d达到峰值;愈创木酚氧化酶在第12 d达产酶高峰,酶活性为713.2 u;多酚氧化酶分别在第12 d与第18 d出现产酶高峰,其酶活峰值分别是660.1 u和1 001. 0 u;胞外多糖含量的最大值(23.70 mg/L)也出现在第12 d.
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| 关 键 词： | 云芝 液体培养 愈创木酚氧化酶 多酚氧化酶 胞外多糖 |
| 文章编号： | 1001-7461(2004)02-0022-03 |
| 修稿时间： | 2003年9月10日 |
Changes of Extracellular Enzyme Activity and Extracellular Polysaccharide During the Submerged Culture of Corilus versicolor |
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| WANG Jun-ming,ZHOU Zi-yun,HU Jing-jiang,CAO Zhi-min.Changes of Extracellular Enzyme Activity and Extracellular Polysaccharide During the Submerged Culture of Corilus versicolor[J].Journal of Northwest Forestry University,2004,19(2):22-24. |
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| Authors: | WANG Jun-ming ZHOU Zi-yun HU Jing-jiang CAO Zhi-min |
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| Institution: | WANG Jun-ming~1,ZHOU Zi-yun~1,HU Jing-jiang~1,CAO Zhi-min~2 |
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| Abstract: | Dynamic variation of extracellular enzyme activity ,extracellular polysaccharide and other relative target during the submerged culture of Corilus versicolor were investigated. The results showed that the maxmum of hypha growth was 6.436 g/L, appeared in the 12th days .There was not significant change of pH of the fluid medium through the whole period of the cultivation. The maxmun of extracellular protein consistencey was 1.06 mg/mL, appeared in the 11th days. The peak of guaicaal oxidase enzyme production appeared in the 12th days, the maxmum enzyme activity was 713.2 u. The peak of enzyme production of polyphenol oxidase appeared twice. The first appeared in the 12th days, and its enzyme activity was 660.1 u. The second appeared in the 18th days, its enzyme activity was 1001.0 u . Extracellular polysaccharide appeared its maxmum in the 12th days, it was 23.70 mg/L. |
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| Keywords: | Corilus versicolor submerged culture guaiacal oxidase polyphenol oxidase extracellular polysaccharide |
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