| 抗病性不同大豆品种根面及根际微生物区系的变化 Ⅰ.非连作大豆(正茬)根面及根际微生物区系的变化 |
| |
| 引用本文： | 陈宏宇,李晓鸣,王敬国.抗病性不同大豆品种根面及根际微生物区系的变化 Ⅰ.非连作大豆(正茬)根面及根际微生物区系的变化[J].植物营养与肥料学报,2005,11(6):804-809. |
| |
| 作者姓名： | 陈宏宇 李晓鸣 王敬国 |
| |
| 作者单位： | 1.中国农业大学资源与环境学院农业部植物营养与养分循环重点实验室教育部植物-土壤相互作用重点实验室 北京100094; |
| |
| 摘 要： | 采用平板计数法测定了3个抗病性不同的大豆品种在生育期内根面和根际微生物区系的变化情况,并应用荧光计数法直接测定了根际细菌和真菌的生物量。结果表明,土体的微生物种类最丰富、根际的次之、根面的较单一。播种后从三叶期到鼓粒初期,根面和根际的可培养细菌总数随生育期逐渐增加,鼓粒初期达最大值,而成熟期则有明显的下降;大豆根际细菌生物量也存在相同的变化规律。抗病性不同的大豆品种其根面、根际可培养细菌总数存在差异;抗病品种大豆的根瘤重明显高于感病品种。种植一季后感病品种根际积累的病原生物(镰孢霉Fusarium.sp.和大豆胞囊线虫Heterodera.glycines的胞囊数)明显高于抗病品种。说明大豆根系分泌物对微生物具有选择性的促进或抑制作用,不同大豆品种以及同一大豆品种在不同生育时期根系分泌物的组成和数量不同,从而使大豆根面及根际形成了特定的微生物区系组成。
|
| 关 键 词： | 大豆 根面 根际 微生物区系 抗病 |
| 文章编号： | 1008-505X（2005）06-0804-07 |
| 收稿时间： | 2004-11-04 |
| 修稿时间： | 2004年11月4日 |
Change of microflora in the rhizoplane and rhizosphere of different disease resistance soybean cultivarsⅠ . Change of microfiora in the rhizoplane and rhizosphere of soybean under normal rotation cropping condition |
| |
| CHEN Hong-Yu,LI Xiao-Ming,WANG Jing-Guo.Change of microflora in the rhizoplane and rhizosphere of different disease resistance soybean cultivarsⅠ . Change of microfiora in the rhizoplane and rhizosphere of soybean under normal rotation cropping condition[J].Plant Nutrition and Fertilizer Science,2005,11(6):804-809. |
| |
| Authors: | CHEN Hong-Yu LI Xiao-Ming WANG Jing-Guo |
| |
| Institution: | 1.College of Resour.and Environ.Sci.;CAU;Key Lab.of Plant Nutrition and Nutrient Cycling;MOA;Key Lab.of Soil-Plant Interaction;MOE;Beijing 100094;China; |
| |
| Abstract: | Previous research found many factors might cause the obstacle,such as the change of physical and chemical properties in the soil,plant allelopathy and soil-borne disease,among which the soybean cyst nematode(SCN) and the soybean root rot(SRR) were the most serious and popular soil-borne diseases.The microflora in the rhizoplane and rhizosphere were affected by the root activity,especially,the root exudates.Differences in component and amount of the root exudates between resistant cultivar and susceptible cultivar hadn't been found.In order to investigate the microflora in the rhizoplane and rhizosphere soil of different disease resistant cultivar,we chose three soybean cultivars,the resistant cultivar(Kangxian 2,K2) to SCN,the resistant cultivar(Suinong 10,S10) to SRR,the susceptible cultivar(Hefeng 25,H25) to both SCN and SRR.A root box container with three compartment was covered by nylon net(30μm) which could limit the plant root going over.the seeds sowed in the middle compartment.Sampling was taken at the trifoliate stage,beginning of flowering stage,early filling stage and harvest maturity stage,respectively.The microflora in the rhizoplane,rhizosphere and bulk soil of the three cultivars was investigated during the growth stage with plate culture method.The biomass of bacteria and fungi in rhizosphere and bulk soil was detected by direct fluorescence microscopic counting.The results showed that the species of microorganisms in bulk were the richest,followed by rhizosphere soil and the microorganism species in rhizoplane were most simplex.The numbers of colony forming units(CFU) of bacteria in rhizoplane and rhizosphere soil increased gradually during the trifoliate stage and the early filling stage,reached a peak at the early filling stage,and then decreased dramatically at the mature stage.The numbers of CFU bacteria in the rhizoplane and rhizosphere soil for different soybean cultivars varied significantly.The root nodule weight of resistant cultivar was significantly higher than that of susceptible one.A more pathogenic microbe(Fusarium sp.and cyst of Heterodera glycines) in the rhizoplane and rhizosphere soil of susceptible cultivar at the mature stage was observed.It indicated that soybean root exudates had certain selective acceleration or inhibition role on the microorganisms in the rhizoplane and rhizosphere soil.The component and amount of root exudates varied between different soybean cultivars and even different growth stage of same soybean cultivar,resulting in the formation of special microflora in the rhizoplane and rhizosphere soil. |
| |
| Keywords: | soybean rhizoplane rhizosphere microflora disease resistance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物营养与肥料学报》浏览原始摘要信息 |
| 点击此处可从《植物营养与肥料学报》下载免费的PDF全文 |
|
