|
|||||
|
|
| 上海地区31株2型猪链球菌的分离鉴定及其耐药性分析 | |
| 引用本文： | 李彩英,岳修伟,侯怡轩,顾江萍,王淑杰,李培锋,杨志彪.上海地区31株2型猪链球菌的分离鉴定及其耐药性分析[J].中国兽医寄生虫病,2011,19(6):54-59. |
| 作者姓名： | 李彩英 岳修伟 侯怡轩 顾江萍 王淑杰 李培锋 杨志彪 |
| 作者单位： | 1. 内蒙古农业大学兽医学院,内蒙古010018 上海交通大学农业与生物学院,上海200240 2. 上海交通大学农业与生物学院,上海,200240 3. 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨,150001 4. 内蒙古农业大学兽医学院,内蒙古,010018 |
| 基金项目： | 兽医生物技术国家重点实验室开放基金 |
| 摘 要： | 猪链球菌病是由猪链球菌引起的一种重要人畜共患病,尤其是猪链球菌2型,不仅对猪的致病性最强,而目可感染特定人群并致死,给人类公共卫生和养猪业带来巨大影响。本研究采集临床症状疑似2型猪链球菌感染猪的全血及内脏器官,通过观察菌落形态及培养特征,筛选出可疑样品,纯化培养并进行PCR鉴定,筛选到31株2型猪链球菌,最后用纸片扩散法对临床分离到的2型猪链球菌菌株测定其耐药性,结果显示对5种药物耐药率分别为：80%（AM）、68%（GM）、84%（CHL）、52%（SXT）、55%（CIP）。试验结果表明分离的31株2型猪源性链球菌,均为多重耐药株,且青霉素类药物的耐药性较为严重,这为临床在治疗2型猪链球菌病提供了合理的指导作用。 |
| 关 键 词： | 2型猪链球菌 PCR鉴定 纸片扩散法 耐药性 |
ISOLATION OF STEPTOCOCCUS SUIS TYPE 2 IN SHANGHAI AREA AND TESTING OF DRUG RESISTANCE |
|
| HOU Yi-xuan,GU Jiang-ping,WANG Shu-jie,Xue-hui,LI Pei-feng,YANG Zhi-biao.ISOLATION OF STEPTOCOCCUS SUIS TYPE 2 IN SHANGHAI AREA AND TESTING OF DRUG RESISTANCE[J].Chinese Journal of Veterinary Parasitology,2011,19(6):54-59. | |
| Authors: | HOU Yi-xuan GU Jiang-ping WANG Shu-jie Xue-hui LI Pei-feng YANG Zhi-biao |
| Institution: | (Agricultura University, Huhhot 010018, China; 2. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China,3. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China) |
| Abstract: | Streptococcus suis infection is an important zoonotic disease. Streptococcus suis type 2 is highly pathogenic to pigs and also can infect humans and cause deaths. Whole blood and internal organs were collected from diseased pigs suspected S. suis type 2 infection. The samples were cultured for examination of colony morphology and growth characteristics and for identification by PCR. A total of 31 strains were determined to be S. suis type 2. The drug resistance of these strains was tested by using disk diffusion method. The percentages of drug resistant strains were 80% to AM, 68% to GM, 84% to CHL, 52% to SXT, and 55% to CIE These results would provide valuable information for clinical treatment of S. suis type 2 infection. |
| Keywords: | Streptococcus suis type2 PCR disc diffusion method drug resistance |
| 本文献已被 维普 万方数据 等数据库收录！ | |