| 鸡包涵体肝炎病毒山西株的分离与鉴定 |
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| 引用本文： | 张坦,郑杰,张发明,孙丰廷,张红,杨国良,龙进学,张亮,王文泉.鸡包涵体肝炎病毒山西株的分离与鉴定[J].中国畜牧兽医,2013,40(4):194-197. |
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| 作者姓名： | 张坦 郑杰 张发明 孙丰廷 张红 杨国良 龙进学 张亮 王文泉 |
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| 作者单位： | 北京华都诗华生物制品有限公司,北京 102600 |
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| 摘 要： | 为了确诊山西某鸡场疑似鸡包涵体肝炎的的病例,本研究进行了鸡包涵体肝炎病毒的分离鉴定。将病死鸡肝脏研磨,经卵黄囊途径接种6日龄SPF鸡胚,成功获得1株病毒;并经过鸡胚病变特征、RT-PCR、基因测序、BLAST分析及动物回归试验等,成功分离出1株鸡包涵体肝炎病毒。进一步证明该病例是鸡包涵体肝炎病毒感染所致。
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| 关 键 词： | 鸡包涵体肝炎 分离 鉴定 山西株 |
| 收稿时间： | 2012-09-27 |
Isolation and Identification of Inclusion Body Hepatitis Virus in Shanxi |
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| ZHANG Tan,ZHENG Jie,ZHANG Fa-ming,SUN Feng-ting,ZHANG Hong,YANG Guo-ling,LONG Jin-xue,ZHANG Liang,WANG Wen-quan.Isolation and Identification of Inclusion Body Hepatitis Virus in Shanxi[J].China Animal Husbandry & Veterinary Medicine,2013,40(4):194-197. |
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| Authors: | ZHANG Tan ZHENG Jie ZHANG Fa-ming SUN Feng-ting ZHANG Hong YANG Guo-ling LONG Jin-xue ZHANG Liang WANG Wen-quan |
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| Institution: | Beijing Ceva Huadu Biological Co.,Ltd.,Beijing 102600,China |
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| Abstract: | The purpose of this study was to evaluate a suspected chicken inclusion body hepatitis virus. A case of suspected chicken inclusion body hepatitis occurred on a chicken farm in Shanxi province. A live suspension culture of deadly suspected chickens was made and inoculated into the yolk cavity of a 6-day old SPF embryo. The virus was then isolated. After further analysis of the infected lesion using RT-PCR, gene sequencing, BLAST search and animal regression testing, it was determined that the suspected culprit was the inclusion body hepatitis virus. |
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| Keywords: | inclusion body hepatitis virus isolation identification Shanxi strain |
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