| 牛磺胆酸对小鼠腹腔巨噬细胞Gsα基因表达的影响 |
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| 引用本文： | 郭文瑞,毛伟,关红,李培锋.牛磺胆酸对小鼠腹腔巨噬细胞Gsα基因表达的影响[J].中国畜牧兽医,2013,40(4):10-13. |
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| 作者姓名： | 郭文瑞 毛伟 关红 李培锋 |
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| 作者单位： | 内蒙古农业大学兽医学院,内蒙古呼和浩特010018;农业部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特010018 |
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| 摘 要： | 本试验旨在探讨牛磺胆酸(taurocholate acid,TCA)对小鼠腹腔巨噬细胞刺激性G蛋白α(Gsα)基因表达的影响,为阐明TCA对小鼠腹腔巨噬细胞的作用机制提供线索。采用荧光定量PCR技术检测TCA对小鼠腹腔巨噬细胞Gsα mRNA表达的影响。试验结果显示,高(15 μg/mL)、中(1.5 μg/mL)和低剂量(0.15 μg/mL)的TCA作用组小鼠腹腔巨噬细胞Gsα mRNA的表达均显著高于对照组和LPS刺激组(P＜0.05)。结果表明,TCA能显著促进小鼠腹腔巨噬细胞Gsα mRNA的表达。
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| 关 键 词： | 牛磺胆酸 刺激性G蛋白α 荧光定量PCR 腹腔巨噬细胞 |
| 收稿时间： | 2012-09-13 |
Effect of TCA on Gsα Expression in Peritoneal Macrophage in Mouses |
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| GUO Wen-rui
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MAO Wei
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GUAN Hong
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LI Pei-feng.Effect of TCA on Gsα Expression in Peritoneal Macrophage in Mouses[J].China Animal Husbandry & Veterinary Medicine,2013,40(4):10-13. |
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| Authors: | GUO Wen-rui MAO Wei GUAN Hong LI Pei-feng |
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| Institution: | 1. College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2. Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease,Ministry of Agriculture,Hohhot 010018,China |
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| Abstract: | The assay was aimed to explore the effect of taurocholate acid (TCA) on Gsα expression in peritoneal macrophage in mouses, and provide clues for explaining the function mechanism of TCA on mouse peritoneal macrophages. The peritoneal macrophages of mouse were isolated and cultured. The effect of TCA on the expression of Gsα mRNA was examined quantitatively by Real-time fluorescent quantitative PCR. TCA(15,1.5 and 0.15 μg/mL) decreased significantly the expression of Gsα mRNA in peritoneal macrophage in mouses (P＜0.05), compared with that in control group and LPS group. The results showed that TCA could significantly promote the expression of Gsα mRNA in peritoneal macrophage in mouses. |
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| Keywords: | taurocholate acid Gsα Real-time fluorescent quantitative PCR peritoneal macrophage |
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