| 薄层层析－紫外分光光度法测定预混剂中的恩拉霉素 |
| |
| 引用本文： | 周鹏飞,陈敏,方中.薄层层析－紫外分光光度法测定预混剂中的恩拉霉素[J].中国畜牧兽医,2013,40(4):125-128. |
| |
| 作者姓名： | 周鹏飞 陈敏 方中 |
| |
| 作者单位： | 1. 浙江工商大学食品与生物工程学院,浙江杭州 310012;2. 浙江凯胜科技有限公司,浙江杭州 310014 |
| |
| 基金项目： | 兽用抗生素研发及剂形研究开发 |
| |
| 摘 要： | 试验旨在建立薄层层析－紫外分光光度法测定预混剂中恩拉霉素的方法。使用硅胶GF254薄层板为固定相,正丁醇∶冰醋酸∶水(2∶1∶1)为展开剂,在紫外光灯(254 nm)下定位,样品中的恩拉霉素得到较好分离。在271 nm波长处对不同含量的恩拉霉素标品进行紫外检测,结果表明,恩拉霉素点样量在20~166 μg范围内与吸光度呈现良好的线性关系(r=0.9989),平均回收率为99.85%,RSD值为3.1%(n=9)。此法所测定的结果与高效液相色谱(HPLC)法所测定的结果基本一致。该方法简单、快捷、准确、分析量大,适用于预混剂中恩拉霉素的定量分析。
|
| 关 键 词： | 恩拉霉素 薄层层析－紫外分光光度法 预混剂 |
| 收稿时间： | 2012-09-20 |
Determination of Enramycin in Premix by TLC-UV Spectrophotometry |
| |
| ZHOU Peng-fei
,
CHEN Min
,
FANG Zhong.Determination of Enramycin in Premix by TLC-UV Spectrophotometry[J].China Animal Husbandry & Veterinary Medicine,2013,40(4):125-128. |
| |
| Authors: | ZHOU Peng-fei CHEN Min FANG Zhong |
| |
| Institution: | 1. College of Food Science & Bioengineering,Zhejiang Gongshang University, Hangzhou 310012,China;2. Zhejiang Chyszern technology Co.,Ltd.,Hangzhou 310014,China |
| |
| Abstract: | A method of TLC-UV was set up for determining enramycin in premix. Target components were extracted by acetone aqueous solution. Using TLC plate, a mixture of N-Butanol∶glacial acetic acid∶water (2∶1∶1) was used as developer. Fixing the position under the light of a UV lamp(254 nm), detection wavelength was 271 nm. The absorbance and quantity of sample application showed a good linear relationship when the quantity of enramycin ranged from 20 to 166 μg (r=0.9989) with average recovery of 99.85% and RSD value of 3.1%(n=9). The content of enramycin in premix detected by this method was almost the same as that was detected by HPLC. This method which was simple, rapid and accurate,large amount of analysis,could be used to the quantitative analysis of enramycin in premix. |
| |
| Keywords: | enramycin TLC-UV spectrophotometry premix |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
