| 滇黄芩器官发生与不同继代次数遗传稳定性的RAPD分析 |
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| 引用本文： | 岑举人,步怀宇,王英娟,赵宇玮,贾敬芬.滇黄芩器官发生与不同继代次数遗传稳定性的RAPD分析[J].广西农业生物科学,2009(2):289-292. |
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| 作者姓名： | 岑举人 步怀宇 王英娟 赵宇玮 贾敬芬 |
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| 作者单位： | 西北大学;西部资源生物与现代生物技术教育部重点实验室; |
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| 基金项目： | 陕西省自然科学基金(2007C104);;陕西省教育厅基金(07JK379)资助 |
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| 摘 要： | 本研究以滇黄芩不带腋芽茎段为外植体，采用不同配比激素的MS培养基建立了滇黄芩器官发生，植株再生体系。结果表明，茎段在MS＋6-BA 2mg／L＋NAA 0.5mg／L培养基上培养时，愈伤组织诱导率达到100％；以相同培养基进行分化，并在MS＋6-BA 2mg／L＋NAA 0.2mg／L培养基上扩繁丛生芽。在1／2MS＋IBA 0.2mg／L培养基诱导生根，扦插继代。利用RAPD分析不同继代次数再生植株，所用的39条随机引物中只有1条引物带型发生变化。说明经组织培养获得的再生植株遗传稳定，可多次继代培养，为滇黄芩进一步遗传操作和扩大药材资源奠定基础。
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| 关 键 词： | 滇黄芩 植物再生 继代次数 遗传稳定 RAPD |
Organogenesis of Scutellaria amoena and Genetic Stability of Regenerated Plantlet Analyzed by RAPD |
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| Cen Juren Bu Huaiyu Wang Yingjuan Zhao Yuwei Jia Jingfen Key Laboratory of Resource Biology , Biotechnology in Western China,Ministry of Education,Northwest University,Xi'an.Organogenesis of Scutellaria amoena and Genetic Stability of Regenerated Plantlet Analyzed by RAPD[J].Journal of Guangxi Agricultural and Biological Science,2009(2):289-292. |
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| Authors: | Cen Juren Bu Huaiyu Wang Yingjuan Zhao Yuwei Jia Jingfen Key Laboratory of Resource Biology Biotechnology in Western China Ministry of Education Northwest University Xi'an |
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| Institution: | Cen Juren Bu Huaiyu Wang Yingjuan Zhao Yuwei Jia Jingfen Key Laboratory of Resource Biology , Biotechnology in Western China,Ministry of Education,Northwest University,Xi'an,710069 |
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| Abstract: | Organogenesis and plant regeneration system was established of Scutellaria amoena C.H.Wright on MS medium with different phytohormones. Non-axillary bud stems fregments were used as the explants. MS medium added with 2 mg/L 6-BA and 0.5 mg/L NAA was optimal for callus induction. The frequency of callus induction reached to 100%. Buds were differentiated on the same medium,and grew up on MS medium with 2 mg/L 6-BA,0.2 mg/L NAA and 20 g/L sucrose. Regenerated shoots were rooted and subcultured on half strengt... |
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| Keywords: | Scutellaria amoena C H Wright Plant regeneration Repeatedly subculturing Genetic stability RAPD |
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