| 假俭草胚性愈伤组织分化及再生植株的变异研究 |
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| 引用本文： | 向佐湘,刘明稀.假俭草胚性愈伤组织分化及再生植株的变异研究[J].中国草地学报,2012,34(1):65-71. |
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| 作者姓名： | 向佐湘 刘明稀 |
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| 作者单位： | 湖南农业大学农学院,湖南 长沙,410128 |
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| 基金项目： | 湖南农业大学校青年基金(10QN30)资助 |
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| 摘 要： | 以假俭草种子为外植体,对假俭草再生体系的持久性、离体再生方式以及再生植株的变异情况进行了初步研究,结果表明假俭草的高效再生体系至少可以维持33个月;体细胞胚发生是其主要再生途径;继代时间超过12个月的胚性愈伤分化的植株出现包括矮化,花叶、花期改变,抗旱增强等的多种变异。
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| 关 键 词： | 假俭草 再生体系 体细胞胚发生 体细胞无性系变异 |
Study on Differentiation of Embryogenic Calli and Somaclonal Variation of Centipedegrass |
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| XIANG Zuo-xiang
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LIU Ming-xi.Study on Differentiation of Embryogenic Calli and Somaclonal Variation of Centipedegrass[J].Chinese Journal of Grassland,2012,34(1):65-71. |
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| Authors: | XIANG Zuo-xiang LIU Ming-xi |
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| Institution: | (College of Agronomy,Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | An efficient and long-term regeneration system is crucial to crop breeding by biotechnology.The persistence of regeneration system of centipedegrass Eremochloa ophiuroides(Munro) Hack],regeneration approach in vitro and variation of the regenerated plantlets were studied by using the centipedegrass seeds as explant.The results showed that the efficient regeneration system can remain 33 months at least,also the main regeneration approach of somatic embryogenesis was confirmed.At the same time,different variations including dwarfing,yellow-strip variation on the leaf,changed flowering period,improved drought-resistance,were observed in the plantlets regenerated from the calli subcultured for 12 months at least. |
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| Keywords: | Centipedegrass Regeneration system Somatic embryogenesis Somaclonal variation |
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