| 辣椒乙烯反应转录因子基因CaJERF1 的克隆及诱导表达 |
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| 引用本文： | 张秋平,杨宇红,茆振川,陈国华,谢丙炎.辣椒乙烯反应转录因子基因CaJERF1 的克隆及诱导表达[J].园艺学报,2012,39(4):705-712. |
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| 作者姓名： | 张秋平 杨宇红 茆振川 陈国华 谢丙炎 |
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| 作者单位： | （1 中国农业科学院蔬菜花卉研究所，北京 100081；2 湖南农业大学，长沙 410128） |
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| 基金项目： | 北京市自然科学基金项目(5062030) |
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| 摘 要： | 应用RT-PCR、RACE技术从乙烯诱导的辣椒中克隆到一个乙烯反应（ERF）类转录因子基因，命名为CaJERF1，其全长cDNA序列为1 379 bp，编码375个氨基酸，分子量为41.5 kD，具有一个由59个氨基酸构成的保守的ERF结构域。CaJERF1的AP2域与CaPF的同源性为100%，与JERF1的同源性为99%；全序列与CaPF和JERF1的同源性也分别达到99%和86%，属于ERF家族的第Ⅳ次家族。诱导分析表达结果表明，植物激素乙烯、苿莉酸甲酯以及盐和低温胁迫均可诱导该基因的表达，表明CaJERF1可能参与到多条信号途径中，并在多种逆境胁迫应答中发挥作用。
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| 关 键 词： | 辣椒 ERF 转录因子 乙烯 植物激素 诱导表达 |
Cloning and Inducible Expression of Ethylene Response Factor CaJERF1 in Hot Pepper |
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| ZHANG Qiu-ping,YANG Yu-hong,MAO Zhen-chuan,CHEN Guo-hua,and XIE Bing-yan.Cloning and Inducible Expression of Ethylene Response Factor CaJERF1 in Hot Pepper[J].Acta Horticulturae Sinica,2012,39(4):705-712. |
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| Authors: | ZHANG Qiu-ping YANG Yu-hong MAO Zhen-chuan CHEN Guo-hua and XIE Bing-yan |
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| Institution: | （1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Hunan Agricultural University，Changsha 410128，China） |
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| Abstract: | An ethylene response factor gene CaJERF1 was isolated from hot pepper using the methods of RT-PCR and RACE. The full length of the cDNA sequence is 1 379 bp,encoding a protein of 375 amino acids,with predicted molecular weight 41.5 kD. The putative protein contains a conserved ERF motif of 59 amino acids that shares 100% and 99% identities with this domain of CaPF and tomato JERF1,respectively. Alignment of full sequences indicates that CaJERF1 protein has high similarity to CaPF(99%)and JERF1(86%),belonging to Ⅳ subgroup of ERF subfamily. Northern blot analyses showed that ethylene,jasmonic acid,high salt and low temperature obviously induced the expression of CaJERF1,suggesting that CaJERF1 might be involved in multiple stress responses. |
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