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AG和AGPs对大白菜和普通白菜(小白菜)小孢子胚状体诱导及成苗的影响
引用本文:王爱杰,章云,刘洋,冯辉.AG和AGPs对大白菜和普通白菜(小白菜)小孢子胚状体诱导及成苗的影响[J].中国蔬菜,2012,1(4):62-66.
作者姓名:王爱杰  章云  刘洋  冯辉
作者单位:沈阳农业大学园艺学院,辽宁省十字花科蔬菜遗传育种重点实验室,辽宁沈阳 110866
基金项目:现代农业产业技术体系建设专项(nycytx-35)
摘    要:以3个耐抽薹大白菜和3个普通白菜(小白菜)一代杂种为试材进行小孢子培养,研究阿拉伯半乳聚糖(AG)和阿拉伯半乳糖蛋白(AGPs)对大白菜和普通白菜小孢子胚状体诱导及其成苗的影响。结果表明:在NLN-13培养基中添加10 mg?L-1AG、10 mg?L-1AGPs或10 mg?L-1 AG+10 mg?L-1 AGPs,可以促进大白菜和普通白菜小孢子胚状体发生及直接成苗。

关 键 词:大白菜  普通白菜(小白菜)  阿拉伯半乳聚糖  阿拉伯半乳糖蛋白  小孢子胚状体  植株再生  

Effect of AG and AGPs on Microspore Embryogenesis and Plant Regeneration of Chinese Cabbage and Pakchoi
WANG Ai-jie,ZHANG Yun,LIU Yang,FENG Hui.Effect of AG and AGPs on Microspore Embryogenesis and Plant Regeneration of Chinese Cabbage and Pakchoi[J].China Vegetables,2012,1(4):62-66.
Authors:WANG Ai-jie  ZHANG Yun  LIU Yang  FENG Hui
Institution:Liaoning Province Key Laboratory of Genetics and Breeding of Cruciferous Vegetable Crops,College of Horticulture,Shenyang Agricultural University,Shenyang 110866,Liaoning,China
Abstract:Effect of AG and AGPs on microspore embryogenesis and plant regeneration was investigated by using 3 late-bolting hybrids of Chinese cabbage(Brassica campestris L.ssp.pekinensis(Lour)Olsson)and 3 hybrids of Pakchoi(B.campestris L.ssp.chinensis(L.)Makino var.communis Tsen et Lee)as material.The results showed that adding 10 mg·L-1 AG,10 mg·L-1 AGPs or 10 mg·L-1 AG+10 mg·L-1 AGPs to the medium of NLN-13 could improve the ability of microspore embryogenesis and support plant regeneration.
Keywords:Chinese cabbage  Pakchoi  AG  AGPs  Microspore embryogenesis  Plant regeneration
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