| 水稻水通道蛋白OsPIP2-6的亚细胞定位研究（摘要） |
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| 引用本文： | 姚清国,李晓芹,周二鹏,王娟,张文娜.水稻水通道蛋白OsPIP2-6的亚细胞定位研究（摘要）[J].农业科学与技术,2011(3):387-388,404. |
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| 作者姓名： | 姚清国 李晓芹 周二鹏 王娟 张文娜 |
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| 作者单位： | 石家庄学院化工学院 |
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| 基金项目： | Supported by Program for Science and Technology Development in Shijiazhuang City(10120803);Doctoral Research Fund of Shijiazhuang University(2007012)~~ |
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| 摘 要： | 目的]探讨水稻水通道蛋白OsPIP2-6的亚细胞定位。方法]克隆了水稻中的重要通道蛋白OsPIP2-6基因,构建了瞬时表达载体,通过基因枪法在洋葱表皮中进行瞬时表达,并通过激光共聚焦显微镜进行了观察。结果]水稻水通道蛋白OsPIP2-6主要定位在细胞质膜上,证明了OsPIP2-6是个水通道蛋白。结论]为植物水通道蛋白的深入研究提供了理论依据。
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| 关 键 词： | 水通道蛋白 水稻 亚细胞定位 |
Subcellular Localization of Aquaporins OsPIP2-6 in Rice |
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| YAO Qing-guo,LI Xiao-qin,ZHOU Er-peng,WANG Juan,ZHANG Wen-na.Subcellular Localization of Aquaporins OsPIP2-6 in Rice[J].Agricultural Science ＆ Technology,2011(3):387-388,404. |
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| Authors: | YAO Qing-guo LI Xiao-qin ZHOU Er-peng WANG Juan ZHANG Wen-na |
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| Institution: | Chemistry Department of Shijiazhuang University Shijiazhuang,Shijiazhuang 050035 |
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| Abstract: | Objective] This study was to explore the subcellular localization of aquaporins OsPIP2-6 in rice. Method] A key rice aquaporins gene OsPIP2-6 was cloned and used for construction of a transient expression vector,which was then transformed into onion epidermis via particle bombardment for confocal microscopy analysis using YFP gene as a reporter gene. Result] The results showed that rice aquaporins OsPIP2-6 was mainly located in the plasma membrane. Conclusion] Our results provided theoretical basis for further understanding plant aquaporins. |
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| Keywords: | Aquaporins Rice Subcellular localization |
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