| 烟草根特异性启动子TobRB7的分离及克隆 |
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| 引用本文： | 曲波.烟草根特异性启动子TobRB7的分离及克隆[J].吉林农业科技学院学报,2008,17(3):9-11. |
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| 作者姓名： | 曲波 |
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| 作者单位： | 吉林农业工程职业技术学院,公主岭136100 |
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| 摘 要： | 从烟草品种“NC89”中提取总DNA，设计特异性引物，用PCR方法分离得到长度为670bp左右的烟草根特异性启动子TobRB7基因，井插入克隆载体pMD18-Tvector，为构建高效的植物表达载体奠定基础。
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| 关 键 词： | 烟草 根特异性启动子TobRB7 PCR 克隆 |
Isolation and Cloning of Root-Specific Promoter TobRB7 from Tobacco |
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| QU Bo.Isolation and Cloning of Root-Specific Promoter TobRB7 from Tobacco[J].Journal Of Jilin Agricultural Science And Technology College,2008,17(3):9-11. |
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| Authors: | QU Bo |
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| Institution: | QU Bo(Jilin Agricultural Engineering Vocational Technology College,Gongzhuling 136100,China) |
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| Abstract: | The total DNA was extracted from tobacco NC89,then around 670bp root-specific promoter was isolated by PCR using the special primers,and inserted into the cloning vector pMD18-T,which laid the foundation for the construction of high efficiency expression vector. |
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| Keywords: | tobacco root-specific promoter TobRB7 PCR cloning |
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