Homocysteine activates Rho kinase signaling pathway to promote viability and migration of rat basilar arterial vascular smooth muscle cells |
| |
| Authors: | XIAO Meng REN Chuan-zhong |
| |
| Institution: | School of Medicine, Xinyang Vocational and Technical College, Xinyang 464000, China |
| |
| Abstract: | AIM:To investigate the regulatory effects of homocysteine (Hcy) on the viability and migration of rat basilar arterial smooth muscle cells (BASMCs) and its potential molecular mechanisms. METHODS:BASMCs were isolated, cultured in vitro and treated with Hcy at different concentrations. The cell viability was measured by CCK-8 assay, and the activation of Rho kinase pathway was measured by Western blot. The cells were treated with Hcy at fixed concentration (1 mmol/L), and ROCK inhibitor Y-27632 was also used. The cell cycle distribution was analyzed by flow cytometry. The cell migration ability was detected by wound healing assay and Transwell assay. The activation of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the level of malondialdehyde (MDA) were measured for determining the status of oxidative stress.RESULTS:Hcy increased the viability of BASMCs and the protein expression of GTP-RhoA and ROCK2 in a dose-dependent manner (P<0.05). Compared with the cells treated with Hcy for 24 h, the cells treated with Hcy for 48 h had enhanced viability (P<0.05). Compared with control group, treatment with Hcy increased cell population in S phase and decreased cell population in G0/G1 phase, while pre-incubation with Y-27632 reversed Hcy-induced G1/S phase transition in BASMCs (P<0.05). The cell migration rate in Hcy treatment group was remarkably higher than that in control group(P<0.05), while pre-incubation with Y-27632 reversed Hcy-induced cell migration (P<0.05). Furthermore, Hcy inhibited the activation of SOD and GSH-Px, accompanied with increased MDA level (P<0.05). Compared with Hcy treatment group, pre-incubation with Y-27632 increased the activation of SOD and GSH-Px, but decreased MDA level (P<0.05). CONCLUSION:Homocysteine induces the viability and migration of rat BASMCs, and its mechanisms may be related to activation of Rho kinase pathway. |
| |
| Keywords: | Homocysteine Basilar arterial smooth muscle cells Cell viability Cell migration Rho kinase signaling pathway |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
