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| 岷县黑裘皮羊微卫星遗传多样性分析 | |
| 引用本文： | 张瑞,郎侠,吴建平,刘婷,宫旭胤,王彩莲,迟浩斌,梁婷玉.岷县黑裘皮羊微卫星遗传多样性分析[J].中国畜牧兽医,2019,46(8):2360-2370. |
| 作者姓名： | 张瑞 郎侠 吴建平 刘婷 宫旭胤 王彩莲 迟浩斌 梁婷玉 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院, 兰州 730070; 2. 甘肃省农业科学院畜草与绿色农业研究所, 兰州 730030 |
| 基金项目： | 甘肃省农业科学院农业科技创新专项学科团队项目（2017GAAS30）;甘肃省现代草食畜产业技术体系："牛羊遗传资源利用岗位"（GARS-08） |
| 摘 要： | 试验旨在分析岷县黑裘皮羊群体内遗传多样性,筛选出理想的遗传标记,为岷县黑裘皮羊的选育保种提供理论依据。选取144只岷县黑裘皮羊,颈静脉采血并提取DNA,对24对微卫星引物进行PCR扩增,用毛细管电泳技术进行基因分型,计算其等位基因数、等位基因大小及频率、有效等位基因数、杂合度和多态信息含量。结果显示,24个位点共检测到210个等位基因,平均等位基因数为8.75;群体等位基因频率为0.0104~0.7396,等位基因片段大小为97~285 bp;有效等位基因数为1.7581~8.2433个;群体平均观测杂合度为0.4839;平均期望杂合度为0.6959;平均多态信息含量为0.6527。其中MAF70位点等位基因数、有效等位基因数、期望杂合度和多态信息含量最高;OarFCB128位点观测杂合度最高;OarAE129位点等位基因数最少,SRCRSP9位点期望杂合度、多态信息含量最低;OarFCB304位点观测杂合度最低。Hardy-Weinberg平衡分析结果表明,所选位点中有19个处于非平衡状态。结果表明,岷县黑裘皮羊的遗传背景复杂,群体内遗传多样性丰富,可为其遗传资源的评估和选育保种工作提供理论依据。 |
| 关 键 词： | 岷县黑裘皮羊 微卫星 遗传多样性 毛细管电泳 |
| 收稿时间： | 2018-12-27 |
Genetic Diversity Analysis of Microsatellite in Minxian Black Fur Sheep |
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| ZHANG Rui,LANG Xia,WU Jianping,LIU Ting,GONG Xuyin,WANG Cailian,CHI Haobin,LIANG Tingyu.Genetic Diversity Analysis of Microsatellite in Minxian Black Fur Sheep[J].China Animal Husbandry & Veterinary Medicine,2019,46(8):2360-2370. | |
| Authors: | ZHANG Rui LANG Xia WU Jianping LIU Ting GONG Xuyin WANG Cailian CHI Haobin LIANG Tingyu |
| Institution: | 1. College of Animal Science & Technology, Gansu Agricultural University, Lanzhou 730070, China; 2. Animal Husbandry, Pasture and Green Agriculture Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730030, China |
| Abstract: | This study was aimed to analyze the genetic diversity of Minxian Black fur sheep, select the ideal genetic markers, and provide a theoretical basis for breeding and conservation.144 Minxian Black fur sheep were selected to collect blood from jugular vein for extracting DNA.24 pairs of microsatellite primers were amplified by PCR, and the genotypes were analyzed by capillary electrophoresis.The numbers of alleles, alleles size and frequency, numbers of effective alleles, heterozygosity and polymorphism information content were calculated.The results showed that a total of 210 alleles were found in 24 loci, the average number of alleles was 8.75;The population alleles frequency were 0.0104 to 0.7396, the alleles size were 97 to 285 bp;The numbers of effective alleles were 1.7581 to 8.2433;The average observed heterozygosity was 0.4839;The average expected heterozygosity was 0.6959;The average polymorphism information content was 0.6527.The numbers of alleles, numbers of effective alleles, expected heterozygosity and polymorphism information content at MAF70 locus were the highest;The observe heterozygosity at OarFCB128 locus were the highest;The numbers of alleles at OarAE129 locus and the observed heterozygosity at OarFCB304 locus were the lowest;The expected heterozygosity and polymorphism information content at SRCRSP9 locus were the lowest.The Hardy-Weinberg equilibrium analysis results showed that 19 loci devieted from Hardy-Weinberg equilibrium.The results indicated that the genetic background of Minxian Black fur sheep was complex and the genetic diversity was rich, which could provide a theoretical basis for the evaluation of genetic resources and the breeding and conservation. |
| Keywords: | Minxian Black fur sheep microsatellite genetic diversity capillary electrophoresis |
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