The Extracellular Enzymes Activity of F1 Generations and Single-spore Isolations in Agrocybe salicacola Strain YAAS711 |
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| Authors: | HuiMing ZHOU YongChang ZHAO HongMei CHAI YanZhen ZHANG |
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| Institution: | 1. School of Biotechnology and Engineering, West Yunnan University, Lincang 677000, Yunnan;2. Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Sciences, Kunming 650205 |
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| Abstract: | 【Objective】 The objective of this study was to explore the changes in activities of extracellular enzymes (EEA) of single-spore isolations and its self-crossing individuals from Agrocybe salicacola YAAS711 and to provide evidence for its genetic breeding. 【Method】 Eighteen single-spore monokaryons and 22 dikaryons from self-crossing progeny of A. salicacola YAAS711 were used as the test materials. The activities of CMCase, filter paper cellulase, β-glucanase, hemicellulase, amylase, protease, laccase, polyphenolase and peroxidase of all test strains were determined by 3,5-dinitrosalicylic acid (DNS), Folin methods and guaiacol method, and correlation analysis were carried out between the 9 EEs with mycelial growth rate, polarities and mating factors.【Results】 The results of 9 EEs showed that most of the both monokaryons and dikaryons had higher activities of amylase, filter paper cellulase, hemicellulose, β-glucanase, CMCase and protease, but their activities of peroxidase, polyphenolase and laccase were low. The mycelial growth rate of monokaryons and dikaryons had a certain degree of correlation with the average activity of 9 EEAs. Compared with the dikaryons derived from the combination of “fast-fast” growth rate of monokaryons, the dikaryons derived from the combination of “fast-slow” growth rates of monokaryons had higher EEAs, except hemicellulase, protease and polyphenol oxidase activities. However, there were no rules to follow, when it came to the combination of dikaryons from the cross of type II monokaryons. The indirect effect of polyphenolase on the mycelial growth rate of monokaryons was greater than that of laccase, but the combined effect of laccase on the mycelial growth rate of monokaryons was the largest. The synergistic effect among EEs had little effect on the mycelial growth rate of dikaryons, among them, laccase had the greatest direct effect on its mycelial growth rate. Multiple comparisons showed that the effect of polarity on EEs was greater than that of mating factors at a significant level of 0.05.【Conclusion】 In short, there was a significant correlation between the anomalies of the mycelial growth rate of the monokaryons from same polarity single spore isolations and the EEAs of F1 generations deriverd from these monokaryons in A. salicacola strain YAAS711. The EEAs of type I strains had same rule, but the differences were existed in some type II monokaryons and the dikaryons. There were certain correlation between the growth rate of monokaryons, EEAs and polarity, there were no direct correlation between mating factors and EEAs. |
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| Keywords: | Agrocybe salicacola self-crossing F1 generations extracellular enzyme activity monokaryon dikaryon |
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