| 油菜抗咪唑啉酮类除草剂基因标记的开发与应用 |
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| 基金项目： | National Key Research and Development Program of China(2016YFD0101300);National Natural Science Foundation of China(31671731);National Natural Science Foundation of China(31901503);China Agricultural Research System(CARS-13);Natural Science Foundation of Jiangsu Province(BK20190267) |
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| 摘 要： | 草害已成为严重制约我国油菜生产的重要因素。种植抗除草剂品种和采用化学除草是防控草害的经济有效途径。为了开展分子标记辅助选择(marker-assisted selection,MAS)育种,加速抗除草剂品种培育,本研究针对已发现的抗咪唑啉酮类除草剂油菜M9中BnALS1R基因编码区第1913位点的SNP变异,开发高通量、低成本的竞争性等位基因特异PCR (kompetitive allele specific PCR, KASP)标记。采用筛选出的KBA1R19681913B标记在2个F_2群体中进行KASP反应。结果表明,该标记能有效检测群体中存在的BnALS1R 3种基因型,其分离比均为1︰2︰1,遵循单基因遗传规律,且基因型与表型完全吻合。将该标记用于BnALS1R抗性纯合基因的回交转育,获得200多个抗咪唑啉酮油菜恢复系。该标记还可在油菜苗期鉴定抗性杂交种的纯度。KASP标记KBA1R19681913B的获得为油菜抗除草剂MAS育种以及抗性新种质的培育提供了技术支撑。
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| 收稿时间： | 2020-03-02 |
Development and application of the marker for imidazolinone-resistant gene in Brassica napus |
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| Authors: | HU Mao-Long CHENG Li GUO Yue LONG Wei-Hua GAO Jian-Qin PU Hui-Ming ZHANG Jie-Fu CHEN Song |
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| Institution: | 1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Sub-center, National Center of Oil Crops Improvement / Key Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture and Rural Affairs / Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences / Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014, Jiangsu, China;2. Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Jiangsu, China |
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| Abstract: | Weed damage is one of the most important factors restricting the development of rapeseed production in China. Cultivation of herbicide-resistant varieties combined with chemical herbicides was an extremely effective help to control field weed in rapeseed production. To accelerate the breeding of cultivars with resistance imidazolinone herbicides through molecular marker-assisted selection in Brassica napus, the simplest, most cost-effective and high-throughput KASP markers were developed by using a SNP mutation at 1913 bp position of ALS (acetolactate synthase) gene in the mutant M9. A polymorphic marker designed KBA1R19681913B was obtained to effectively distinguish the homozygous resistant M9 from susceptible rapeseeds. Meanwhile, the KASP marker was evaluated in two F2 populations and could effectively discriminate three genotypes. F2 populations gave a good fit to the expected 1:2:1 ratio, confirming a single gene Mendel model with the perfect matched between phenotyping and genotyping. About more than two hundred homozygous restoring lines containing BnALS1R were developed through KASP marker selection in multi-generation backcross and showed stable imidazolinone herbicide resistance. This functional marker can also be used to identify the purity of hybrid seeds at the seedling stage. In conclusion, the validated KASP marker KBA1R19681913B would provide a technical support for developing herbicide-resistant rapeseed in marker-assisted selection and identification of resistant germplasm. |
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| Keywords: | weed damage rapeseed (Brassica napus L ) marker-assisted selection imidazolinone herbicides KASP marker |
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