Total flavonoids of onion attenuate hydrogen peroxide-induced oxidative damage in retinal pigment epithelial cells |
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| Authors: | YAO Xian-feng ZHENG Kai-jin CHEN Mei WANG Li-min |
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| Institution: | Ophthalmology Department, First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China |
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| Abstract: | AIM: To investigate the effects of total flavonoids of onion (FO) on hydrogen peroxide (H2O2)-induced oxidative damage in retinal pigment epithelial cells. METHODS: The retinal pigment epithelium ARPE-19 cells were divided into 5 groups:control group, H2O2 group (treated with H2O2), FO-L+H2O2 group (treated with H2O2 and low concentration of FO), FO-M+H2O2 group (treated with H2O2 and medium concentration of FO) and FO-H+H2O2 group (treated with H2O2 and high concentration of FO). The cell viability was measured by MTT assay. Apoptosis was analyzed by flow cytometry. DCFH-DA staining was used to detect reactive oxygen species (ROS) level in the cells. WST assay was used to detect superoxide dismutase (SOD) activity. The content of malonaldehyde (MDA) was measured by TBA method. Mitochondrial membrane potential was analyzed by JC-1 staining. The protein levels of cytochrome C (Cyt C) in the cytoplasm, and cleaved caspase-3 and cleaved caspase-9 in the cells were determined by Western blot. RESULTS: Treatment with H2O2 decreased ARPE-19 cell viability, increased the apoptotic rate and the level of ROS in the cells, decreased SOD activity, increased the content of MDA, decreased mitochondrial membrane potential, and increased the protein levels of Cyt C in the cytoplasm and cleaved caspase-3 and cleaved caspase-9 in the cells (P<0.05). Compared with H2O2 group, the cell viability in FO-L+H2O2 group, FO-M+H2O2 group and FO-H+H2O2 group was increased, the apoptotic rates were decreased, the levels of ROS were decreased, SOD activity was increased, the content of MDA was decreased, mitochondrial membrane potential was increased, the protein level of Cyt C was decreased in the cytoplasm, and the protein levels of cleaved caspase-3 and cleaved caspase-9 protein in the cells were decreased gradually (P<0.05). CONCLUSION: Total flavonoids of onion reduce H2O2-induced oxidative damage in retinal pigment epithelial cells. |
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| Keywords: | Retinal pigment epithelial cells Total flavonoids of onion Oxidative damage Mitochondrial membrane potential |
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