Effects of ZIP14 on biological behaviors of hepatocellular carcinoma cell line BEL-7404 |
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| Authors: | ZHENG Jia-ying LI Ya-dong ZHENG Qing-zhu YU Li-li WU Qing-wei QIU Fu-nan WU Yan-an HUANG Yi |
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| Institution: | 1.Provincial College ofClinical Medicine, Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, China;2.Department of Clinical Laboratory, Fujian Provincial Hospital, Fujian Provincial Hospital, Fuzhou 350001, China;3.Department of Hepatobiliary Surgery, Fujian Provincial Hospital, Fuzhou 350001, China |
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| Abstract: | AIM: To study the expression of zinc transporter ZRT/IRT-like protein 14 (ZIP14) in the hepatocellular carcinoma (HCC) tissues, and to investigate the effects of ZIP14 over-expression on the biological behaviors of HCC cells. METHODS: The expression of ZIP14 at mRNA and protein levels in the HCC tissues and adjacent non-tumor tissues were detected by real-time PCR and immunohistochemical staining, respectively. The lentivirus expression system containing GV365-ZIP14 was constructed, and was used to infect the HCC cell line BEL-7404, which had relatively poor expression of ZIP14. The expression of ZIP14 at mRNA and protein levels in the transfected cells were detected by real-time PCR and Western blot, respectively. Under the conditions of zinc sulfate stimulation at different concentrations, the cell viability, the cell cycle, and the cell migration and invasion abilities were detected by MTT assay, DNA ploid detection, and Transwell assay, respectively. RESULTS: The mRNA expression level and the strong-positive rate of protein expression of ZIP14 in the HCC tissues were significantly lower than those in the adjacent non-tumor liver tissues (P<0.01). The expression of ZIP14 at mRNA and protein levels in the BEL7404 cells was significantly enhanced by infection of GV365-ZIP14 expression lentivirus. Compared with negative control group (transfected with negative control lentivirus), the cell viability, migration and invasion in ZIP14 over-expression group (transfected with GV365-ZIP14 expression lentivirus) were significantly reduced, and the percentage of the cells in G2/M phase was significantly increased, all of which were more obvious with the elevation of zinc concentration in the culture medium. CONCLUSION: ZIP14 is low expressed in the HCC tissues. The ZIP14 over-expression has inhibitory effects on the viability, migration and invasion of HCC cells, and blocks the cell cycle in G2/M phase, which might be closely related to the elevation of zinc concentration in cytoplasma of HCC cells due to enchanced zinc transport by ZIP14. |
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| Keywords: | Hepatocellular carcinoma Zinc transporters ZRT/IRT-like protein |
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