| 利用RIL群体创制低山嵛酸花生新种质 |
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| 摘 要： | 花生是我国食用植物油的重要来源,改善花生油脂品质是我国花生育种的重要目标。利用不同遗传背景的亲本(中花10号×ICG12625)杂交构建含有140个家系的重组自交系群体(RIL),对超长链饱和脂肪酸(花生酸、山嵛酸和二十四碳烷酸)含量测定表明, RIL家系超长链饱和脂肪酸含量的变异范围为4.27%～7.05%,均值为5.54%,最低值分别比父本和母本低1.46和1.63个百分点,降低率达25.27%和27.62%;山嵛酸含量的变异范围为1.86%～3.37%,均值为2.53%, 3份家系的山嵛酸含量稳定低于2%(QT0002、QT0075和QT0120),比低值亲本中花10号分别低0.57、0.51和0.51个百分点,降低率分别为23.45%、20.98%和20.98%。利用前期构建的遗传图谱,通过WinQTLcart软件检测到一个与山嵛酸相关的稳定的QTL,位于B04染色体13.31～16.34 M内,该区间内含有131个预测基因。本结果为高油酸花生品种的山嵛酸遗传改良奠定了基础。
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| 收稿时间： | 2019-08-14 |
Novel peanut genotype with low behenic acid developed from recombinant inbred lines |
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| Authors: | Jian-Bin GUO Li HUANG Nian LIU Huai-Yong LUO Xiao-Jing ZHOU Wei-Gang CHEN Bei WU Dong-Xin HUAI Xiao-Ping REN Hui-Fang JIANG |
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| Institution: | Oil Crops Research Institute, China Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China |
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| Abstract: | Peanut is an important source of edible vegetable oil in China, and improving the quality of peanut oil is an important goal in peanut breeding. In this study, the contents of very long-chain saturated fatty acids (arachidic acid, behenic acid, and tetracosanoic acid) were determined by using RIL population containing 140 lines derived from the cross between Zhonghua 10 and ICG12625 with different genetic backgrounds. The content of very long- chain saturated fatty acid in the RILs was 4.27%-7.05%, with an average of 5.54%, the minimum value was 1.46 and 1.63 percentage points lower than that of male and female parents, with reduction rates of 25.27% and 27.62%, respectively. The behenic acid content ranged from 1.86% to 3.37%, with an average of 2.53%. Three lines of QT0002, QT0075, and QT0120 had behenic acid content lower than 2%, which was 0.57, 0.51, and 0.51 percentage points lower than that of Zhonghua10 with the reduction rate of 23.45%, 20.98%, and 20.98%, respectively. A stable QTL located in 13.31-16.34 M of B04 was detected using a linkage map and WinQTLcart software. In this region of 13.31-16.34 M contained 131 prediction genes. The results lay a foundation for the genetic improvement of low behenic acid and high-oleic peanut varieties. |
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| Keywords: | peanut recombined inbreed lines (RILs) fatty acid behenic acid |
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