miR-422a regulates SOX6 to inhibit injury of PC12 cells induced by hydrogen peroxide |
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| Authors: | ZHANG Guang-hua TIE Ting-ting JU Hu XU Chang-lin XIAO Zong-yu YUAN Le |
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| Institution: | 1. Department of Neurosurgery, Affiliated Hospital of Qinghai University, Xining 810001, China;
2. Department of Neurology, Affiliated Hospital of Qinghai University, Xining 810001, China;
3. Teaching Management Department, Affiliated Hospital of Qinghai University, Xining 810001, China |
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| Abstract: | AIM: To investigate the effects of microRNA-422a (miR-422a) on the damage of rat adrenal gland pheochromocytoma PC12 cells induced by hydrogen peroxide (H2O2). METHODS: The expression of miR-422a in the PC12 cells treated with H2O2 was detected by real-time PCR. After miR-422a mimics were transfected into PC12 cells, the cell viability was measured by MTT assay, the lactate dehydrogenase (LDH) leakage rate was detected, and the apoptosis was analyzed by flow cytometry. Target gene prediction software was used to predict that sex-determining region Y box 6 (SOX6) may be the target gene of miR-422a. Luciferase reporter assay was used to identify the targeting relationship. miR-422a mimics and SOX6 over-expression vector were co-transfected into the PC12 cells. The effects of SOX6 over-expression on the viability, LDH leakage rate and apoptosis of PC12 cells treated with H2O2 and transfected with miR-422a mimics were evaluated. RESULTS: The expression of miR-422a in the PC12 cells was decreased after treatment with H2O2 (P<0.05). The viability of PC12 cells treated with H2O2 was decreased, and the LDH leakage rate and apoptotic rate were increased. Transfection with miR-422a mimics enhanced the viability of PC12 cells treated with H2O2, and the leakage rate of LDH and apoptotic rate of the PC12 cells were reduced. The expression of SOX6 was negatively regulated by miR-422a. SOX6 over-expression reversed the effects of miR-422a on PC12 cell viability, LDH leakage and apoptosis. CONCLUSION: miR-422a reduces the damage of PC12 cells induced by H2O2 via targeting SOX6. |
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| Keywords: | MicroRNA-422a PC12 cells SOX6 gene Apoptosis Cell viability |
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