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添加不同酶制剂对全株玉米青贮发酵品质的影响
引用本文:张适,吴琼,尤欢,杨浩,胡宗福,王思珍,牛化欣.添加不同酶制剂对全株玉米青贮发酵品质的影响[J].中国畜牧兽医,2019,46(4):1045-1052.
作者姓名:张适  吴琼  尤欢  杨浩  胡宗福  王思珍  牛化欣
作者单位:1. 内蒙古民族大学动物科学技术学院, 通辽 028000;
2. 内蒙古自治区肉牛疾病防控工程技术研究中心, 通辽 028000;
3. 通辽市家畜繁育指导站, 通辽 028000
基金项目:内蒙古自治区"草原英才工程"青年创新创业人才第一层次培养项目(NM2017-22):内蒙古牧草益生乳酸菌资源的发掘及其青贮饲料在肉牛TMR中的应用研究;内蒙古自治区自然基金面上项目(2018MS03022):全株玉米青贮有氧暴露诱发梭菌发酵机理及控制研究;内蒙古自治区自然基金项目:基于宏基因组技术研究全株玉米青贮对科尔沁肉牛瘤胃微生物区系的影响(2018LH03013);内蒙古民族大学研究生科研立项:具有抑制梭菌的益生乳酸菌对全株玉米青贮发酵品质的影响(NMDSS1862)
摘    要:本研究旨在探讨添加不同酶制剂对全株玉米青贮发酵品质的影响,以蜡熟期刈割的全株玉米为材料,设对照组和6个处理组,处理组分别添加5 g/kg纤维素酶、木聚糖酶、果胶酶、α-半乳糖苷酶、β-甘露聚糖酶和β-葡聚糖酶,每组3个重复,青贮56 d后对全株玉米青贮饲料的感官评定、发酵品质、营养成分进行比较分析。结果显示:纤维素酶组、木聚糖酶组和β-葡聚糖酶组全株玉米青贮饲料的青贮效果较好;与对照组相比,纤维素酶组、木聚糖酶组和β-葡聚糖酶组可显著提高青贮饲料碳水化合物(WSC)和乳酸的含量(P>0.05),同时使青贮氨态氮/总氮显著降低(P<0.05),青贮发酵品质较好;添加纤维素酶的全株玉米青贮中干物质(DM)含量高于对照组和其他处理组,添加纤维素酶和α-半乳糖苷酶处理的粗蛋白质(CP)含量较高;添加纤维素酶的酸性洗涤纤维(ADF)和β-葡聚糖酶组青贮中性洗涤纤维(NDF)、ADF均显著低于对照组(P<0.05)。综上表明:纤维素酶、木聚糖酶和β-葡聚糖酶更适于用作全株玉米青贮饲料的添加剂。

关 键 词:全株玉米  青贮  酶制剂  纤维素  可溶性碳水化合物(WSC)
收稿时间:2018-09-13

Effects of Different Enzyme Preparations on Fermentation Quality of Whole Corn Silage
ZHANG Shi,WU Qiong,YOU Huan,YANG Hao,HU Zongfu,WANG Sizhen,NIU Huaxin.Effects of Different Enzyme Preparations on Fermentation Quality of Whole Corn Silage[J].China Animal Husbandry & Veterinary Medicine,2019,46(4):1045-1052.
Authors:ZHANG Shi  WU Qiong  YOU Huan  YANG Hao  HU Zongfu  WANG Sizhen  NIU Huaxin
Institution:1. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China;
2. Inner Mongolia Autonomous Region Beef Disease Prevention and Control Engineering Research Center, Tongliao 028000, China;
3. Tongliao City Livestock Breeding Guidelines Station, Tongliao 028000, China
Abstract:The purpose of this study was to investigate the effects of different enzyme preparations on fermentation quality of whole corn silage to improve its utilization efficiency.The whole corn that castrating during wax maturity were used as materials and assigned into 7 groups which were control group and 6 treatment groups with 3 repeats per group.The six treatment groups were added 5 g/kg cellulase,xylanase,pectinase,α-galactosidase,β-mannanase and β-glucanase,respectively.Then the sensory evaluation,fermentation quality and nutrient composition of different processed corn silage were compared after 56 d of silage.The results showed that the whole corn in cellulase,xylanase and β-glucanase groups had better silage effect on sensory evaluation.Compared with control group,cellulase,xylanase and β-glucanase could significantly increase carbohydrate and lactic acid contents in whole corn silage (P>0.05),and significantly reduce ammonia nitrogen/total nitrogen (P<0.05),so that the silage had a significant improvement in fermentation quality.The DM content in cellulase group was higher than that of control group and other treatment groups.The content of CP treated with cellulase and α-galactosidase was higher;The ADF content in cellulase group and contents of NDF and ADF in β-glucanase group were significantly lower than that in control group (P<0.05).In summary,under the experimental condition,cellulase,xylanase and β-glucanase were more suitable as additives for whole corn silage.
Keywords:whole corn  silage  enzyme preparations  cellulose  water soluble carbohydrates(WSC)  
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