| 利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性 |
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| 基金项目： | This study was supported by the National Key Research and Development Program of China (2017YFE0105100);the Crop Germplasm Resources Protection(2019NWB036-07);the Agricultural Science and Technology Innovation Program (ASTIP) in CAAS, and the Fundamental Research Funds for Central Non-Profit of Institute of Crop Sciences, CAAS.(S2018XC04) |
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| 摘 要： | 利用基于窄叶羽扇豆转录组开发并筛选出的95对多态性EST-SSR标记,对羽扇豆属的22个种133份资源进行全基因组扫描,初步探究羽扇豆属下种间的进化关系,分析来源于"旧世界"羽扇豆种间的遗传多样性,为羽扇豆优异资源的挖掘和创新利用提供理论依据。结果表明,用95对SSR标记共检测出1318个等位变异,每对标记平均检测出3～37个等位变异,平均为13.87个;多态性信息量(PIC)变化范围0.39～0.91,平均为0.75;基因多样性变化范围0.41～0.92,平均为0.78。基于邻接法(NJ)的系统发育初步探究了羽扇豆种间的进化关系,22个羽扇豆种分别来源于"旧世界"和"新世界",与之前研究结果相对一致。聚类分析、群体结构分析和主成分分析结果均表明,来源于"旧世界"的7个羽扇豆种被划分为4个组群,各组群所包含的参试资源没有种间交叉重叠。
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| 收稿时间： | 2019-05-21 |
Assessment of genetic diversity by using EST-SSR markers in Lupinus |
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| Authors: | Hong-Yan ZHANG Tao YANG Rong LIU Fang JIN Li-Ke ZHANG Hai-Tian YU Jin-Guo HU Feng YANG Dong WANG Yu-Hua HE Xu-Xiao ZONG |
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| Institution: | 1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / The National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;2. National Agro-Tech Extension & Service Centre, Beijing 100125, China;3. Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China;4. USDA-ARS Western Regional Plant Introduction Station (WRPIS), Pullman, WA 99164, USA |
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