| 荧光蛋白标记研究进展 |
| |
| 引用本文： | 杨晓玫,姚拓,师尚礼.荧光蛋白标记研究进展[J].草业学报,2019,28(10):209-216. |
| |
| 作者姓名： | 杨晓玫 姚拓 师尚礼 |
| |
| 作者单位： | 甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃 兰州 730070;甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃 兰州 730070;甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃 兰州 730070 |
| |
| 基金项目： | 省委组织部“微生物肥料关键技术及新产品研发”(LYRC2019-113)资助 |
| |
| 摘 要： | 荧光蛋白标记由于其独特的颜色感官,在医学、遗传育种和草地植被抗病中占有重要的位置。荧光蛋白分子标记的选择与方法与其特异性的DNA有关,基因的结构是荧光蛋白标记、进行基因交流和促进基因表达的重要部分。荧光蛋白标记技术主要包括热激转导和电击转化两种不同的类型,需通过大肠杆菌作为载体结合菌株,不影响其任何生物功能,是目前大分子示踪检测及研究定殖的主要方法,研究荧光蛋白标记对病原菌和根际促生菌的定殖和良好利用优良菌株有着重要意义。但是,荧光蛋白标记是在分子水平,对研究的方法增加了难度。根据目前国内外已有的部分荧光蛋白标记研究,评述了标记的原理,标记的适用性以及标记的不同方法等内容,并展望了不同标记方法对利用荧光蛋白的可能性,以期为研究荧光蛋白标记提供系统的方法参考和技术借鉴。
|
| 关 键 词： | 荧光蛋白 分子标记 标记方法 适用性 展望 |
| 收稿时间： | 2019-01-04 |
| 修稿时间： | 2019-04-01 |
Progress in fluorescent protein labeling |
| |
| YANG Xiao-mei,YAO Tuo,SHI Shang-li.Progress in fluorescent protein labeling[J].Acta Prataculturae Sinica,2019,28(10):209-216. |
| |
| Authors: | YANG Xiao-mei YAO Tuo SHI Shang-li |
| |
| Institution: | 1.Key Laboratory of Grassland Ecosystem, Ministry of Education, College of Pratacultural Science, Gansu Agricultural University, Lanzhou 730070, China; 2.Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China |
| |
| Abstract: | Fluorescent protein labeling is an important method for research in the fields of medicine, genetic breeding, and grassland vegetation resistance. The selection of, and methods for using, fluorescent protein molecular markers are related to their specific DNA-binding properties. Gene structure affects fluorescent protein labeling, gene communication, and gene expression. Fluorescent protein labeling technology involves two types of transformation; heat shock and electric shock transformation. In both methods, Escherichia coli is used as a carrier of the gene construct encoding the fluorescent protein label. Monitoring of fluorescent protein labels is the main method used for macromolecular tracer detection and for observing colonization. Studies involving fluorescent protein labeling have been used for monitoring the colonization of pathogens and rhizosphere bacteria and for optimizing the use of excellent strains. However, it is difficult to study fluorescent protein labeling because the interactions occur at the molecular level. In this paper, we review fluorescent protein labeling in terms of its principles, the applicability of various markers, and different methods of using markers based on published literature. Various fluorescent protein labeling methods are also discussed. This review provides a reference for further studies on fluorescent protein labeling in China. |
| |
| Keywords: | fluorescent protein molecular marker marking method applicability prospect |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《草业学报》浏览原始摘要信息 |
| 点击此处可从《草业学报》下载免费的PDF全文 |
|
