Fine Mapping of the Fuzzless Gene n2 in Cotton |
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| Authors: | Li Simin Zuo Dongyun Cheng Hailiang Zhang Youping Wang Qiaolian Liu Ke Feng Xiaoxu Geng Hongwei Song Guoli |
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| Institution: | 1.Xinjiang Agricultural University, Urumqi 830052, China; 2. Institute of Cotton Research of Chinese Academy of Agricultural Sciences/ State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China |
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| Abstract: | Objective] Cotton fibers are single cells derived from the ovule epidermis, cotton fuzz of seeds are also formed by the ovule epidermis. Therefore, we conduct this experiment to locate the fuzzless gene n2 so as to lay a foundation for its clone and function study. Method] Two F2 populations derived from a cross between the Xinhai 21 and the fuzzless mutant of upland cotton (Gossypium hirsutum L.) n2 was constructed. Combined with cotton genomic data and developed simple sequence repeat markers to locate the gene. The predicted gene sequence was acquired and the differences in its expression in two parents were analyzed by using the quantitative real time polymerase chain reaction method. Result] The fuzzless gene was located in a 181 kbp region on chromosome A12 within markers P61 and Z10. There are seven candidate genes in the region. Quantitative real time polymerase chain reaction verification shows significant differences in the expression of 72098 gene in two parents. Conclusion] In this experiment, the fine mapping and candidate genes analysis of gene n2 were completed, which laid a foundation for n2 gene cloning and functional verification. |
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| Keywords: | Upland cotton fiber fuzzless mutant fine mapping expression analysis |
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