| CRISPR/Cas9技术及其在猪基因编辑中的应用 |
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| 引用本文： | 刘思远,卢丹,唐中林.CRISPR/Cas9技术及其在猪基因编辑中的应用[J].畜牧兽医学报,2020,51(3):409-416. |
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| 作者姓名： | 刘思远 卢丹 唐中林 |
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| 作者单位： | 1. 中国农业科学院深圳农业基因组研究所, 岭南现代农业科学与技术广东省实验室深圳分中心, 农业部农业基因数据分析重点实验室, 深圳 518120;2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;3. 湖南农业大学, 长沙 410128;4. 云南农业大学, 昆明 650201 |
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| 基金项目： | 国家转基因重大专项（2016ZX08006002-005）；深圳市技术攻关重点项目（JSGG20180507182028625） |
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| 摘 要： | CRISPR/Cas9（clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9）是一种新型基因编辑系统，是由细菌和古细菌对抗入侵病毒及外源DNA的适应性免疫防御系统演变而来的。该系统具有简易、精准、经济和高效的优点，目前已广泛应用于基因编辑及其相关研究中。本文总结了近几年CRISPR/Cas9技术在猪基因组编辑中的研究进展，并展望了其应用前景。
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| 关 键 词： | CRISPR/Cas9 基因编辑 猪 应用 |
| 收稿时间： | 2019-08-28 |
Research Progress on CRISPR/Cas9 and Its Application in Pigs Genome Editing |
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| LIU Siyuan,LU Dan,TANG Zhonglin.Research Progress on CRISPR/Cas9 and Its Application in Pigs Genome Editing[J].Acta Veterinaria et Zootechnica Sinica,2020,51(3):409-416. |
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| Authors: | LIU Siyuan LU Dan TANG Zhonglin |
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| Institution: | 1. Genome Analysis Laboratory of Ministry of Agriculture, Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China;2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;3. Hunan Agricultural University, Changsha 410128, China;4. Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) is a novel editing system, which is originally developed from the bacteria defense immune mechanism against viruses infection and plasmid transfer. As a simple, accurate, economical and efficient tool, this technology has been extensively used in the gene-editing and related research fields. The progress and application of CRISPR/Cas9 technology in pig genome editing in the past few years are summarized in this review, and also discusses its future perspectives in pig breeding and clinical trials. |
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| Keywords: | CRISPR/Cas9 genome editing pig application |
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