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| 抗菌肽BSN-37的抑菌活性及其稳定性分析 | |
| 引用本文： | 张炜,杭柏林,司素锦,宁春妹,李杰,胡建和.抗菌肽BSN-37的抑菌活性及其稳定性分析[J].中国畜牧兽医,2019,46(1):287-295. |
| 作者姓名： | 张炜 杭柏林 司素锦 宁春妹 李杰 胡建和 |
| 作者单位： | 1. 河南科技学院动物科技学院, 新乡 453003; 2. 河南省新乡市畜产品质量监测检验中心, 新乡 453003 |
| 基金项目： | 河南省自然科学基金项目（182300410031）;河南省高校科技创新团队支持计划（15IRTSTHN）;河南科技学院高层次人才启动项目（2014020）;河南科技学院大学生创新训练项目（2018CX88）;河南科技学院大学生"百农英才"创新项目（BNYC2017-2-17） |
| 摘 要： | 试验旨在研究抗菌肽BSN-37的抑菌活性和稳定性。采用微量倍比稀释法测定抗菌肽BSN-37的最小抑菌浓度(MIC),菌落计数法分析抗菌肽BSN-37对大肠杆菌CVCC1568的杀菌动力学特征,扩散法测定抗菌肽BSN-37的盐离子稳定性、酸碱稳定性、血清稳定性、胰酶稳定性、热稳定性、反复冻融稳定性、室温保持稳定性、药效保持时间稳定性。结果显示,抗菌肽BSN-37对革兰氏阴性菌(大肠杆菌和沙门氏菌)的MIC为1.5625～25μg/mL,对革兰氏阳性菌(枯草芽孢杆菌和短小芽孢杆菌)的MIC为1.5625～12.5μg/mL,对真菌(白色念珠菌)没有活性;抗菌肽BSN-37可在90min内完全杀灭大肠杆菌CVCC1568;除了Fe2+和胰酶会影响抗菌肽BSN-37的抑菌活性外,在75～100℃的高温、150～250mmol/L高浓度盐离子(Na+、K+、Ca2+和Mg2+)、20%～25%饱和浓度的Cu2+、pH 4～10、20%～25%的血清、10～12次的反复冻融、10～12d室温保存等条件下仍能保持较好的抑菌活性,药效保持时间可达7.5d。本试验结果表明,抗菌肽BSN-37具有替代抗生素成为新抗菌药物的潜力,为抗菌肽BSN-37的临床应用提供了理论依据。 |
| 关 键 词： | 抗菌肽BNS-37 抑菌活性 稳定性 理化特性 杀菌动力学 |
| 收稿时间： | 2018-07-27 |
Bacteriostatic Activity and Stability Analysis of Antimicrobial Peptide BSN-37 |
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| ZHANG Wei,HANG Bolin,SI Sujin,NING Chunmei,LI Jie,HU Jianhe.Bacteriostatic Activity and Stability Analysis of Antimicrobial Peptide BSN-37[J].China Animal Husbandry & Veterinary Medicine,2019,46(1):287-295. | |
| Authors: | ZHANG Wei HANG Bolin SI Sujin NING Chunmei LI Jie HU Jianhe |
| Institution: | 1. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China; 2. Xinxiang Center of Monitor and Inspection for Quality of Livestock Production, Xinxiang 453003, China |
| Abstract: | The aim of the experiment was to study the bacteriostatic activity and stability of the antimicrobial peptide BSN-37.The minimum inhibitory concentration (MIC) of BSN-37 against several bacteria were detected with serial dilution method.The bactericidal dynamics of BSN-37 against Escherichia coli (E.coli) CVCC1568 were measured by colony counting method.The salt ion stability,acid-alkali stability,serum stability,pancreatic enzyme stability,thermal stability,repeated freeze-thaw stability,maintain stability at room temperature,and efficacy stabilization stability were analyzed through diffusion method.The results showed that the MIC of the antimicrobial peptide BSN-37 against Gram negative bacteria (E.coli and Salmonella) was 1.5625 to 25 μg/mL,and the MIC of BSN-37 against Gram positive bacteria (Bacillus subtilis and Bacillus pumilus) were 1.5625 to 12.5 μg/mL.But BSN-37 had no bacteriostatic activity against fungi (Candida albicans).The antimicrobial peptide BSN-37 could completely kill E.coli CVCC1568 within 90 min.In addition to the Fe2+ and pancreatic enzymes could affect the bacteriostatic activity of antimicrobial peptide BSN-37,at other conditions including the high temperature (75 to 100℃),150 to 250 mmol/L high concentration of salt ions (Na+,K+,Ca2+ and Mg2+) and 20% to 25% of saturation concentration of Cu2+,pH 4 to 10,20% to 25% of serum,10 to 12 times of repeated freezing and thawing,preservation for 10 to 12 days at room temperature,BSN-37 could still maintain good bacteriostatic activity.Efficacy maintain time of BSN-37 was more than 7.5 days.The results provided a theoretical basis for the clinical application of antimicrobial peptide BSN-37 which had a potential to replace antibiotics as new antimicrobial agents. |
| Keywords: | antibacterial peptide BSN-37 bacteriostatic activity stability physical and chemical properties bactericidal dynamics |
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