Extraction of Cotton Fiber DNA and Its Application in Traceability |
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| Authors: | Tian Xinquan Fu Xiaoqiong Shi Meng Fang Dan Xu Shuangjiao Ma Lei |
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| Institution: | State Key Laboratory of Cotton Biology/ Laboratory of Quality and Safety Risk Assessment for Cotton Products, Ministry of Agriculture and Rural Affairs/ Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, Henan 455000，China |
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| Abstract: | Objective] To address the questions of lacking monitoring system for origins of varieties, this study explored a DNA extraction method suitable for cotton fiber, aiming to establish an identification system that uses cotton fiber DNA as a medium to trace the authenticity and cultivar of cotton varieties. Method] By improving and optimizing the extraction method, the DNAs of cotton fibers at different days post anthesis and lint from different years were extracted and used as templates for routine polymerase chain reaction (PCR) amplification. 13 pairs of simple sequence repeat(SSR) primers were selected for SSR-PCR amplification using the DNA of Lu 1127, Shikang 126 and Ruiza 816. Result] The DNA extraction method can extract cotton fiber DNAs of different developmental stages and different years. With the development of fiber, although the extracted DNA content is reduced, it can still meet the needs of downstream experiments. The extracted cotton fiber DNAs can be subjected to conventional PCR amplification, the PCR amplified bands were clear; 13 pairs of cotton SSR primers were used for SSR-PCR amplification of cotton fiber DNAs of Lu 1127, Shikang 126 and Ruiza 816, and a clear polymorphic band could be amplified and easily distinguished. Conclusion] This extraction method is suitable for extracting genomic DNA of cotton fiber, and is satisfied with conventional PCR amplification with SSR markers. It is confirmed that the cotton fiber DNA molecular markers are feasible for tracing the source of cotton varieties, and it is expected to provide technical support for ensuring the safety of cotton industry. |
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| Keywords: | cotton fiber DNA SSR molecular marker varieties traceability |
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