Hemagglutinating Activity of Duck Tembusu Virus |
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| Authors: | WANG XiaoLei LIU YueHuan DUAN HuiJuan LIU LiXin YANG ZhiYuan ZHAO JiCheng PAN Jie LIU RuiHua ZHAO WenQi TIAN FangJie Lü JinBao LIN Jian |
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| Institution: | 1. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097;2. Beijing University of Agriculture, Beijing 102206;3. Inner Mongolia Agricultural University, Hohhot 010018 |
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| Abstract: | 【Objective】 The objective of this paper was to study the hemagglutinating activity of duck Tembusu virus (DTMUV). 【Method】The DTMUV was inoculated intracerebrally in the 1-3-day-old sucking mice of BALB/c in different dilution to observe the morbidity. The brains of sucking mice were collected and purified according to the referenced methods. The purified virus fluid was inactivated by suitable inactivator, followed by the inactivated inspection in 6-day-old SPF chicken embryo. The virus fluid was evaluated the hemagglutinating activity with the erythrocyte suspension of chicken, duck, goose, pigeon and swine. The concentration of the erythrocyte suspension used in the hemagglutination test was compared to select the proper concentration. The pH value of the reaction system between 6.0-7.0 and the reaction temperature including 4℃, room temperature and 37℃ were compared. 【Result】 In the 6 day after inoculation , the sucking mice inoculated with 1:10 dilution appeared severe clinical symptoms including twitch, paralysis, and most of them were in agonal stage, while the symptoms of the sucking mice inoculated with 1:50 and 1:100 dilution were slighter. The purified DTMUV fluid was pinkey and clarified. It could be inactivated by formaldehyde but not β-propiolactone that would produce lots of flocks and change the property of the fluid. The DTMUV could hemagglutinate with the erythrocyte suspension of chicken, duck, goose, pigeon and swine, and the agglutination graphics was clear and stable when the concentration of the erythrocyte suspension was 0.33%. The hemagglutinating activity of DTMUV could be showed when the reaction system pH value was 6.0-6.8, while the highest hemagglutination titer appeared during the pH 6.0-6.2. Furthermore, the hemagglutinating activity could be appeared at 4℃, room temperature and 37℃, respectively, although the time required for hemagglutination at 4℃ was much longer. 【Conclusion】The DTMUV proliferated in the sucking mice of BALB/c had a broad spectrum of hemagglutinating activity that could hemagglutinate with the erythrocyte suspension of chicken, duck, goose, pigeon and swine. The hemagglutinating activity was stable and could hemagglutinate with the goose erythrocyte at 4℃, room temperature and 37℃. The suitable reaction conditions were 0.33% concentration of the erythrocyte suspension and pH 6.0-6.2 of the reaction system. |
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| Keywords: | duck Tembusu virus hemagglutinating activity reaction conditions |
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