Proton-sensing receptor OGR1 mediates inhibitory effects of acidic environment on viability and tube formation ability of endothelial progenitor cells |
| |
| Authors: | XU Ji DING Sheng-long CHEN Fang-yi ZHANG Ji-hong GUI Ke-ke XIONG Min LI Bing ZHAO Ming-dong |
| |
| Institution: | 1. Department of Orthopaedics, Fudan University, Shanghai 201508, China;
2. Center Laboratory, Jinshan Hospital, Fudan University, Shanghai 201508, China;
3. Qingpu Branch of Zhongshan Hospital, Fudan University, Shanghai 200032, China |
| |
| Abstract: | AIM: To analyze the expression of ovarian cancer G-protein-coupled receptor 1 (OGR1), a proton-sensing receptor, in the endothelial progenitor cells (EPCs), and to explore the role of OGR1 in acid-regulated viability and tube formation ability of EPCs. METHODS: The method of FITC-UEA-I and DiI-Ac-LDL double staining was used to identify the EPCs. The expression of OGR1 in mouse EPCs was analyzed by real-time-PCR and Western blot. After treatment with different pH media, the OGR1 expression was analyzed in EPCs. The viability and cell cycle distribution of the EPCs were analyzed by CCK-8 and flow cytometry assay. The migration and vascularization of EPCs were measured by scratch test, Transwell migration assay and tube formation experiments after silencing OGR1 using small interfering RNA(siRNA). RESULTS: The isolated endothelial progenitor cells were well differentiated, and FITC-UEA-I and DiI-Ac-LDL staining was positive. The expression of OGR1 at mRNA and protein levels was observed in mouse EPCs. The expression of OGR1 was increased gradually with the decrease of pH value of the medium, while the expression of OGR1 was the highest in the medium of pH 6.4 (P<0.05). pH 6.4 medium inhibited the viability of the EPCs and induced cell cycle arrest at G0/G1 phase. Knock-down of OGR1 expression by siRNA partially reversed the effect of acidic environment on the EPCs (P<0.05). The abilities of migration and tube formation of EPCs were inhibited by pH 6.4 medium, while transfection of siRNA to silence OGR1 expression partially reversed the effect (P<0.05). CONCLUSION: The expression of OGR1 in EPCs is positive, and OGR1 mediates the effects of acid on the viability, migration and tube formation ability of EPCs. |
| |
| Keywords: | Avascular necrosis of the femoral head Ovarian cancer G-protein-coupled receptor 1 Endothe-lial progenitor cells Acidic environment Tube formation ability |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
