Effect of proline-spirooxindole on viability and apoptosis of non-small-cell lung cancer A549 cells |
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| Authors: | MA Jin-zhu YAN Liang ZAN Jia-wei XU Lei WANG Yi ZHANG Gen-bao |
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| Institution: | 1. Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Wuhu 241002, China;
2. School of Life Sciences, Anhui Normal University, Wuhu 241002, China;
3. School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210023, China |
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| Abstract: | AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human non-small-cell lung cancer A549 cells. METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay. The apoptosis was analyzed by flow cytometry. The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of mTOR were determined by Western blot. RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L), the cell viability was decreased (P<0.01) compared with DMSO control group. The apoptotic rate was increased compared with DMSO control group (P<0.01). The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of mTOR was inhibited (P<0.01). CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of mTOR. |
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| Keywords: | Non-small-cell lung cancer Proline-spirooxindole Cell viability Apoptosis |
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