| 中药牛皮消多糖体外抗新城疫病毒作用的研究 |
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| 引用本文： | 布合丽倩穆·依明,赛福丁·阿不拉,艾克拜尔·依明,胡安古丽·努尔别克,阿克珠利·努尔包,李昊.中药牛皮消多糖体外抗新城疫病毒作用的研究[J].中国畜牧兽医,2019,46(9):2737-2744. |
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| 作者姓名： | 布合丽倩穆·依明 赛福丁·阿不拉 艾克拜尔·依明 胡安古丽·努尔别克 阿克珠利·努尔包 李昊 |
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| 作者单位： | 新疆农业大学动物医学学院, 乌鲁木齐 830052 |
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| 基金项目： | 国家自然科学基金项目（31360625） |
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| 摘 要： | 为探讨牛皮消多糖（CAP）对体外抗新城疫病毒（NDV）能力的影响，本试验用水提醇沉法提取牛皮消多糖，通过苯酚硫酸法和红外光谱对牛皮消多糖进行检测，采用MTT法测定牛皮消多糖对鸡胚成纤维细胞（CEF）的安全浓度，多糖的安全浓度统一为625 μg/mL，选择安全浓度范围内的（39.06~625 μg/mL）5种浓度的各级多糖，用3种加药方式（先加多糖后接种病毒、先接种病毒后加多糖、多糖和病毒感作后加药）检测牛皮消多糖对NDV的阻断、抑制及直接杀灭作用。结果显示，先接病毒后加多糖组和多糖与病毒感作后加药组CAP50、CAP30、CAPt、CAP80和CAP70均能显著抑制NDV感染CEF的能力，两组各级多糖抑制率分别为131.23%、110.12%、108.15%、100.24%、93.07%和61.76%、43.73%、44.51%、29.02%、37.45%；先加多糖后接病毒组CAP50、CAP30能显著抑制NDV感染CEF的作用（P<0.05），阻断作用下对NDV的抑制率可达到15.82%、8.33%，其余各组在安全浓度范围内对NDV无抑制作用。各组中药牛皮消多糖均有较好的抗NDV活性，其中对NDV的抑制作用和直接杀灭作用强于对其阻断作用。CAP50及CAP30的抗NDV活性较强，可作为进一步研究材料并具备一定的研究价值。
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| 关 键 词： | 牛皮消多糖 鸡胚成纤维细胞 新城疫病毒 抗病毒 |
| 收稿时间： | 2019-02-26 |
Study on Antiviral Effect of Polysaccharides from Chinese Medicine Cynanchum auriculatum on Newcastle Disease Virus in vitro |
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| BUHELIQIANMU·Yiming,SAIFUDING·Abula,AIKEBAIER·Yiming,HUANGULI·Nuerbieke,AKEZHULI·Nuerbao,LI Hao.Study on Antiviral Effect of Polysaccharides from Chinese Medicine Cynanchum auriculatum on Newcastle Disease Virus in vitro[J].China Animal Husbandry & Veterinary Medicine,2019,46(9):2737-2744. |
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| Authors: | BUHELIQIANMU·Yiming SAIFUDING·Abula AIKEBAIER·Yiming HUANGULI·Nuerbieke AKEZHULI·Nuerbao LI Hao |
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| Institution: | College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | In order to investigate the effect of Cynanchum auriculatum polysaccharide (CAP) on the resistance to Newcastle disease virus (NDV) in vitro,the polysaccharides from Cynanchum auriculatum was extracted by water and alcohol precipitation,phenol sulfuric acid and infrared spectroscopy were used to detect CAP.The safe concentration of CAP on chicken embryo fibroblast (CEF) was determined by MTT method,the safe concentration of CAP was set up to 625 μg/mL,and 5 concentrations in the safe concentration range (39.06 to 625 μg/mL) were selected.Each CAP and NDV were added with three kinds of dosing methods (add CAP and then inoculate the virus,inoculate the virus and then add CAP,CAP and the virus-sensing) to detect the blocking,inhibition and direct effect of CAP on NDV.The results showed that CAP50,CAP30,CAPt,CAP80 and CAP70 could significantly inhibit the ability of NDV to infect CEF after the virus was first added group and the CAP and the virus-sensing group.The inhibition rates of CAP50,CAP30,CAPt,CAP80 and CAP70 were 131.23%,110.12%,108.15%,100.24%,93.07% and 61.76%;43.73%,44.51%,29.02% and 37.45%,respectively.The addition of CAP50 and CAP30 in CAP followed by the virus group could significantly inhibited NDV (P<0.05) with 15.82% and 8.33% inhibition rate under the blocking condition,and the other groups had no inhibitory effect on NDV within the safe concentration range.In conclusion,the CAP had better anti-NDV activity,and the inhibition and direct killing effect on NDV was stronger than the blocking effect.CAP50 and CAP30 had stronger anti-NDV activity and could be used as research materials to study further. |
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| Keywords: | Cynanchum auriculatum polysaccharide chicken embryo fibroblast (CEF) Newcastle disease virus (NDV) antivirus |
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