| 猪瘟病毒核酸检测国家级能力验证结果分析 |
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| 引用本文： | 徐璐,张乾义,夏应菊,王震,李翠,邹兴启,王琴,赵启祖.猪瘟病毒核酸检测国家级能力验证结果分析[J].畜牧兽医学报,2020,51(8):1949-1955. |
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| 作者姓名： | 徐璐 张乾义 夏应菊 王震 李翠 邹兴启 王琴 赵启祖 |
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| 作者单位： | 中国兽医药品监察所, 国家/OIE猪瘟参考实验室, 北京 100081 |
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| 基金项目： | 国家重点研发计划（2016YFD0500702；2018YFD0500801） |
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| 摘 要： | 对猪瘟病毒核酸的检测是实验室检测猪瘟感染的主要手段。为全面考察动物疫病检测机构对猪瘟病毒核酸的检测能力，国家认证认可监督管理委员会（以下简称“认监委”）组织了A类能力验证项目“猪瘟诊断检测技术”（CNCA-19-A01），该项目由中国兽医药品监察所国家/OIE猪瘟参考实验室（以下简称：参考实验室）承担。参考实验室负责制备了检测样品，并进行随机编号。向每个参试实验室发放4份待检盲样，不限定检测方法，参试实验室对各个盲样进行定性判断，并向参考实验室提供检验结果及原始检验报告。出现“不满意”结果的单位可参加补测一次，若补测结果仍为“不满意”，则表明该实验室暂不具备该参数的检测能力。参加本次猪瘟病毒核酸检测的实验室共有97家，初测满意率为93.81%，初测和补测的总体满意率为98.97%。此次能力验证结果表明，我国90%以上猪瘟检测机构能够提供正确的检测结果，可以满足猪瘟诊断和监测的需求。对不达标机构，通过本次能力验证项目查找出了存在的问题，帮助该单位及时纠正错误，进一步提升猪瘟诊断能力。
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| 关 键 词： | 猪瘟病毒 核酸检测 能力验证 |
| 收稿时间： | 2020-02-17 |
Result Analysis of National Proficiency Testing Program for the Detection of Classical Swine Fever Virus Nucleic Acid |
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| XU Lu,ZHANG Qianyi,XIA Yingju,WANG Zhen,LI Cui,ZOU Xingqi,WANG Qin,ZHAO Qizu.Result Analysis of National Proficiency Testing Program for the Detection of Classical Swine Fever Virus Nucleic Acid[J].Acta Veterinaria et Zootechnica Sinica,2020,51(8):1949-1955. |
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| Authors: | XU Lu ZHANG Qianyi XIA Yingju WANG Zhen LI Cui ZOU Xingqi WANG Qin ZHAO Qizu |
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| Institution: | National/OIE Reference Laboratory for Classical Swine Fever, China Institute of Veterinary Drug Control, Beijing 100081, China |
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| Abstract: | Nucleic acid detection is the main method to diagnose the infection of classical swine fever virus (CSFV). To monitor performance and compare the measuring results of CSFV nucleic acid obtained by different laboratories, Certification and Accreditation of the People's Republic of China organized the national proficiency testing program “Diagnostic Techniques for Classical Swine Fever” (CNCA-19-A01). This program was undertaken by National/OIE Reference Laboratory for Classical Swine Fever (NRLCSF) which is affiliated to China Institute of Veterinary Drug Control. Positive and negative samples were prepared and numbered randomly. Four samples were sent to each participant and measured according to the participant's instruction. All participants should submit the qualitative results and the original reports to NRLCSF. If the results are not in line with expectation, the participant would have another chance to retest. If the result was still incorrect, the participant would be considered unqualified to detect CSFV nucleic acid. Ninety-seven institutions have participated this program. The accurate rate is 93.81% for the first test, and 98.97% for the first and second tests. This program shows that over 90% institutions have the capability for CSFV nucleic acid detection. These institutions could provide correct results and meet the requirements for CSF diagnosis and surveillance. For the unqualified institutions, the program could help them to investigate the reason for disagreement and implement their deficiencies. |
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| Keywords: | classical swine fever virus nucleic acid detection proficiency testing |
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